Which scientists gave the details of the techniques of preservation?
  • a) Jacob and Monod
  • b) Kirsop and Doyle
  • c) William and Wilkins
  • d) Boliver and Rodriguez
Which of the following is NOT the technique of preservation?
  • a) storage on agar slants
  • b) storage under liquid nitrogen
  • c) dried cultures
  • d) storage in water
The preservation of agar slopes has an expiration of 6 months and the agar needs to be changed every 6 months. Which of the following can be used to extent sub-culturing to 1 year?
  • a) Paraffin oil
  • b) DMSO
  • c) Glycerol
  • d) Loamy Soil
What is the temperature of liquid nitrogen (°C)?
  • a) -150°C
  • b) -122°C
  • c) -194°C
  • d) -196°C
Which of the following is NOT a cryoprotective agent?
  • a) DMSO
  • b) Glycerol
  • c) Ethylene Glycol
  • d) Paraffin wax
The preservation by liquid Nitrogen is called as ___________
  • a) Cryopreservation
  • b) Lyophilization
  • c) Freeze-drying
  • d) Desiccation
Which of the following method involves sublimation of cell water?
  • a) Cryopreservation
  • b) Lyophilization
  • c) Dried Culture
  • d) Desiccation
“Each batch of newly preserved cultures should be routinely checked to ensure their quality”. Who outlined the statement?
  • a) Lincoln
  • b) Snell
  • c) Malik
  • d) Pridham
Which of the following is/are used in dried culture preservation?
  • a) Sand
  • b) Soil
  • c) Sand and Soil
  • d) Slits
The cultures grown on agar slopes may be stored in the refrigerator or freezer at 5°C to -20°C.
  • a) True
  • b) False
Which of the following is NOT the advantage of lyophilization?
  • a) Dissolution of product
  • b) Processed in aseptic conditions
  • c) Handling and processing time increases
  • d) Do not use heat
The cryoprotective agents are used to prevent cell damage.
  • a) True
  • b) False
During the preservation of microbial cell culture ______
  • a) metabolism stops
  • b) metabolism continues
  • c) metabolism changes
  • d) physiology changes
Which of the following method of preservation is more suitable and used widely?
  • a) Dried Cultures
  • b) Lyophilization
  • c) Salt Storage
  • d) Storage in liquid Nitrogen
Which of the following is not the composition of N-agar plates/ slants?
  • a) Yeast/Beef extract
  • b) Peptone
  • c) NaCl
  • d) Dextrose
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