Q.1
T protein is required for replication. It is a product of ____________
  • a) delayed early transcription
  • b) late transcription
  • c) early transcription
  • d) both early and late replication
Q.2
Vaccinia virus is a commonly used virus and it is a member of pox virus family. It has a large genome of _____________ and can accept at least ____________ of foreign DNA.
  • a) 200kb, 25kb
  • b) 1000kb, 200kb
  • c) 500kb, 50kb
  • d) 800kb, 100kb
Q.3
Vaccinia virus produces its own ______ which is functional in _____ of infected cells where the virus also replicates.
  • a) DNA polymerase, nucleus
  • b) DNA polymerase, cytoplasm
  • c) RNA polymerase, cytoplasm
  • d) RNA polymerase, nucleus
Q.4
It is beneficial to use retrovirus for genetic manipulation of cells in the culture.
  • a) True
  • b) False
Q.5
LNSX is a retrovirus and is combined with ______ in order to create a shuttle vector.
  • a) eukaryotic plasmid
  • b) prokaryotic plasmid
  • c) either eukaryotic or prokaryotic plasmid
  • d) yeast plasmid
Q.6
The retroviral transfected DNA is infected into mammalian cells by helper retrovirus. Helper retrovirus should not be packaged and the method adopted for this is?
  • a) Deletion of the packaging signal in the helper genome
  • b) Deletion of multiple parts of the helper genome
  • c) Division of the helper genome into several pieces
  • d) All of the mentioned
Q.7
Choose the incorrect statement in respect to MACs.
  • a) They are mammalian or human artificial chromosomes
  • b) Large stretches of DNA (in excess of 100 kb) can be inserted
  • c) A selectable marker is required
  • d) It can be maintained for long term
Q.8
Centromere is the most important component of the human artificial chromosome. It is constructed from tandemly repeated copies ofbp of ______ DNA sequence.
  • a) alpha satellite
  • b) beta satellite
  • c) satellite 2
  • d) satellite 1
Q.9
Constitutive promoters are majorly obtained from ________
  • a) fungi
  • b) bacteria
  • c) mammalian cells
  • d) viral cells
Q.10
In naturally occurring bacterial systems, tetracycline binding repressor protein binds in the _____ of tetracycline to the control sequence and _______ transcription.
  • a) presence, activates
  • b) absence, activates
  • c) absence, inactivates
  • d) presence, inactivates
Q.11
Tetracycline binding repressor protein when fused to transcription activator protein from herpes simple virus, then it is called as transactivator protein (tTA).
  • a) True
  • b) False
Q.12
Expression of tTA gene is ________ by doxycycline which is _______ analogue.
  • a) activated, tetracycline
  • b) inactivated, chloramphenicol
  • c) activated, chloramphenicol
  • d) inactivated, tetracycline
Q.13
Glucorticoid-responsive element is ______ promoter from ______ of mouse mammory tumour virus (MMTV).
  • a) inducible, short terminal repeat region
  • b) inducible, long terminal repeat region
  • c) constitutive, short terminal repeat region
  • d) constitutive, long terminal repeat region
Q.14
There are a group of promoters from the genes for metallothioneins. These are a group of proteins rich in ______ residues.
  • a) cysteine
  • b) methionine
  • c) either cysteine or methionine
  • d) cysteine and methionine both
Q.15
The amount of expression of DNA inserted into a vector _______ on promoter and ______ on chromosomal location.
  • a) depends only on, not depends
  • b) depends, depends also
  • c) doesn’t depends, but depends
  • d) neither depends, nor depends
0 h : 0 m : 1 s