Q.1
Choose the incorrect statement for second DNA strand synthesis.
  • a) The RNA: DNA complex is treated with the enzyme RNaseH and DNA pol
  • b) The enzyme RNaseH is responsible for nicking the RNA strand and leaving free 3’ hydroxyl ends
  • c) These RNA fragments can be used as primers
  • d) There is no portion of RNA left attached to the DNA strand
Q.2
What is the final product of the RNaseH method?
  • a) blunt ended dsDNA
  • b) staggered dsDNA at both ends
  • c) staggered dsDNA at 3’ end
  • d) staggered dsDNA at 5’ end
Q.3
What would not happen if the RNA strand is completely removed from RNA: DNA hybrid?
  • a) There are no chances of the synthesis of the second DNA strand
  • b) Chance complementarity would take place
  • c) Hairpin structure would be formed
  • d) Hairpin structure is formed is not the final structure
Q.4
The loop region is single stranded. It can be cleaved by using which enzyme?
  • a) Exonuclease
  • b) S1 nuclease
  • c) RNaseH
  • d) DNase
Q.5
Choose the correct statement with respect to the self priming method of cDNA synthesis.
  • a) It is less preferred than RNaseH method
  • b) A hairpin structure is formed with guarantee
  • c) The sequence corresponding to the 5’ end is lost
  • d) Reverse transcriptase is not used
Q.6
Choose the incorrect statement for the method homopolymer tailing.
  • a) The first step is the RNA: DNA hybrid synthesis
  • b) Terminal transferase is used for the addition of nucleotides on 3’ end
  • c) Terminal transferase adds only at DNA strands
  • d) The DNA strand is now having known sequence at 3’ end
Q.7
Choose the correct statement for RACE.
  • a) It stands for Random Amplification of cDNA ends
  • b) It is for cloning particular cDNA ends
  • c) It is only of one type, which is 5’ RACE
  • d) Sequence data is not available in any case
Q.8
The first primer in the case ofRACE is ___________
  • a) internal sequence
  • b) oligo-dT adaptor molecule
  • c) oligo-dA adaptor molecule
  • d) adaptor oligo-dT primer
Q.9
The first cDNA strand inRACE is tailed with oligo-dA tail.
  • a) True
  • b) False
Q.10
What is the second primer in the case ofRACE?
  • a) Internal primer
  • b) Oligo-dA sequence
  • c) Adaptor-oligo-dT primer
  • d) Oligo-dT adaptor molecule
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