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Genetic Engineering
Genetic Engineering Multiple Choice
Quiz 1
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Q.1
Choose the incorrect statement for second DNA strand synthesis.
a) The RNA: DNA complex is treated with the enzyme RNaseH and DNA pol
b) The enzyme RNaseH is responsible for nicking the RNA strand and leaving free 3’ hydroxyl ends
c) These RNA fragments can be used as primers
d) There is no portion of RNA left attached to the DNA strand
Q.2
What is the final product of the RNaseH method?
a) blunt ended dsDNA
b) staggered dsDNA at both ends
c) staggered dsDNA at 3’ end
d) staggered dsDNA at 5’ end
Q.3
What would not happen if the RNA strand is completely removed from RNA: DNA hybrid?
a) There are no chances of the synthesis of the second DNA strand
b) Chance complementarity would take place
c) Hairpin structure would be formed
d) Hairpin structure is formed is not the final structure
Q.4
The loop region is single stranded. It can be cleaved by using which enzyme?
a) Exonuclease
b) S1 nuclease
c) RNaseH
d) DNase
Q.5
Choose the correct statement with respect to the self priming method of cDNA synthesis.
a) It is less preferred than RNaseH method
b) A hairpin structure is formed with guarantee
c) The sequence corresponding to the 5’ end is lost
d) Reverse transcriptase is not used
Q.6
Choose the incorrect statement for the method homopolymer tailing.
a) The first step is the RNA: DNA hybrid synthesis
b) Terminal transferase is used for the addition of nucleotides on 3’ end
c) Terminal transferase adds only at DNA strands
d) The DNA strand is now having known sequence at 3’ end
Q.7
Choose the correct statement for RACE.
a) It stands for Random Amplification of cDNA ends
b) It is for cloning particular cDNA ends
c) It is only of one type, which is 5’ RACE
d) Sequence data is not available in any case
Q.8
The first primer in the case ofRACE is ___________
a) internal sequence
b) oligo-dT adaptor molecule
c) oligo-dA adaptor molecule
d) adaptor oligo-dT primer
Q.9
The first cDNA strand inRACE is tailed with oligo-dA tail.
a) True
b) False
Q.10
What is the second primer in the case ofRACE?
a) Internal primer
b) Oligo-dA sequence
c) Adaptor-oligo-dT primer
d) Oligo-dT adaptor molecule
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