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Quiz 1
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Q.1
In case of transcription which is the rate limiting step?
a) Binding of RNA polymerase
b) Unwinding of DNA duplex
c) Promoter escape
d) Formation of the open complex
Q.2
The site where repressor binds the DNA is the ______________
a) Promoter
b) Terminator
c) Operator
d) ORF
Q.3
Recruitment of RNA polymerase to the promoter is a ___________
a) Covalent binding
b) Cooperative binding
c) Protein-protein interaction
d) Van der Waals forces
Q.4
The proteins of which of the following genes are regularly needed for cellular activity?
a) Regular genes
b) Smart genes
c) Structural genes
d) Housekeeping genes
Q.5
Which of the following are the products of the constitutive gene?
a) i and ii
b) ii and iii
c) iii and iv
d) i, ii, iii and iv
Q.6
Which of the following is not an example of allosteric regulation?
a) Inactivation of nitrogenase by ADP ribosylation
b) Catabolite repression by CAP in E. coli
c) Regulation of phosphofructokinase activity
d) Regulation of lac operon by allolactose
Q.7
Operons are ___________
a) of approximately uniform size
b) found in some eukaryotes
c) not able to bind to proteins
d) smaller in lower eukaryotes and longer in higher eukaryotes
Q.8
Which of the following are correct according to operons?
a) i and ii
b) ii and iii
c) iii and iv
d) i, ii, iii and iv
Q.9
Choose the correct pair among the following.
a) The DNA sequence to which stigma factor binds – enhancer
b) The DNA sequence to which RNA polymerase binds – operator
c) The DNA sequence that codes for a protein – structural gene
d) The DNA sequence to which repressors binds – promoter
Q.10
Which of the following is not present in the β-galactosidase structural gene and thus is not transcribed by the same promoter?
a) Lac A
b) Lac I
c) Lac Z
d) Lac Y
Q.11
Glucose, as the carbon source, is the first choice by bacteria even if other sugars are available. The mechanism behind this selectivity is ___________
a) Operon repression
b) Glucose utilization
c) Enzyme repression
d) Catabolite repression
Q.12
The lac operon is regulated by cAMP by ___________
a) Binding to lac repressor
b) Binding to operator
c) Binding to promoter
d) Binding to catabolite activator protein
Q.13
Tryptophan operon in E. coli is a repressible operon.
a) True
b) False
Q.14
Which of the following is true about tryptophan operon?
a) Tetramer of identical subunits
b) The RNA product is very stable
c) The Trp repressor binds to the tryptophan
d) The Trp repressor is the product of the Trp operon
Q.15
The expression of the tryptophan operon is independent of the availability of tryptophan in culture media.
a) True
b) False
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