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Q.1
Genetic engineering has been successfully used for producing ... [ CBSE 2010]
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a) Transgenic cow, Rosie, which produces high fat milk for making ghee
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b) Animal like bulls for farm work as they have super power
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c) Transgenic mice for testing safety of polio vaccine before use in humans
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d) Transgenic models for studying new treatments for certain cardiac diseases
Explanation
Transgenic mice are being used to test the safety of the polio vaccine. In 1997, the first transgenic cow, Rosie, produced human protein-enriched milk (2.4 grams per litre). The milk contained the human alpha-lactalbumin and was nutritionally a more balanced product for human babies than natural cow-milk. Answer : (c)
Q.2
SCID is caused by defective gene coding for enzyme ...[ kerala 2006]
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a) Adenosine deaminase
0%
b) Guanosine deaminase
0%
c) Guanosine transferase
0%
d) Adenosine transamine
Explanation
SCID is the most severe form of primary immunodeficiencies. The first clinical gene therapy was given in 1990 to a 4-year old girl with adenosine deaminase (ADA) deficiency. This enzyme is crucial for the immune system to function. The disorder is caused due to the deletion of the gene for adenosine deaminase. In some children ADA deficiency can be cured by bone marrow transplantation; in others it can be treated by enzyme replacement therapy, in which functional ADA is given to the patient by injection. Answer : (a)
Q.3
Chemical knives/molecular scissors of DNA are ...[ MHTCET 2008]
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a) Ligases
0%
b) Transcriptase
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c) Polymerases
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d) Restriction endonucleases
Explanation
Restriction nucleases is also known as molecular scissors as they cut any DNA fragment at specific position. Restriction endonucleases make cuts at specific positions within the DNA. Answer : (d)
Q.4
An improved variety of transgenic basmati rice ... [ CBSE 2010]
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a) Gives high yield and is rich in vitamin A
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b) Is completely resistant to all insects and diseases of paddy
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c) Gives high yield but has no characteristic aroma
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d) Does not require chemical fertilizers and growth hormones
Explanation
Golden rice is an improved variety of transgenic basmati rice, which gives high yield and rich in vitamin A. Answer : (a)
Q.5
The bacterium Bacillus thuringiensis is widely used in contemporary biology as ...[ CBSE 2009]
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a) Source of industrial enzyme
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b) Insecticide
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c) Indicator of water pollution
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d) Agent for production of dairy products
Explanation
Bacillus thuringiensis (Bt) is a species of bacteria that lives in soil. It makes proteins that are toxic to some insects when eaten, but not others. The proteins are not toxic to humans because, like all mammals, we cannot activate them. Bt is not toxic to non-target wildlife. Answer : (b)
Q.6
First hormone produced artificially by culturing bacteria is ... [ AFMC 2009]
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a) Insulin
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b) Adrenaline
0%
c) Thyroxine
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d) Testosterone
Explanation
Human insulin is the synthetic insulin which is grown in the laboratories. Human insulin is laboratory created by growing insulin proteins within E.coli bacteria (Escherichia coli). Answer : (a)
Q.7
Plasmids are used in genetics engineering because they are ...[ CBSE 2000 ]
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a) Easily available
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b) Able to replicate
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c) Able to integrate with host chromosome
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d) Inert
Explanation
Plasmid DNA act as vectors to transfer the piece of DNA attached to it. plasmids and bacteriophages have the ability to replicate within bacterial cells independent of the control of chromosomal DNA. Answer : (b)
Q.8
First clinical application of gene therapy was used in 1992 over a four years old girl for ... [ AIIMS 2011]
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a) Adenosine deficiency
0%
b) Adenine deficiency
0%
c) Growth deficiency
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d) Adenosine deaminase deficiency
Explanation
The first clinical gene therapy was given in 1990 to a 4-year old girl with adenosine deaminase (ADA) deficiency. This enzyme is crucial for the immune system to function. The disorder is caused due to the deletion of the gene for adenosine deaminase. Answer : (d)
Q.9
Removal and insertion of gene is
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a) Genetic engineering
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b) Biotechnology
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c) Gene therapy
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d) Cytogenetics
Explanation
Genetic engineering (also called genetic modification) is a process that uses laboratory-based technologies to alter the DNA makeup of an organism. This may involve changing a single base pair (A-T or C-G), deleting a region of DNA or adding a new segment of DNA. Answer : (a)
Q.10
Which one is a wrong statement .....[ Kerala 2007]
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a) Transposons were visualized by Barbara Mc-Clintock
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b) Ian Wilmut produced cloned sheep Dolly
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c) In pedigree analysis, a person immediately affected is called propositus
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d) DNA ligases are used to cleave DNA molecule
Explanation
When cut by the same restriction enzyme, the resultant DNA fragments have the same kind of ‘sticky-ends’ and, these can be joined together (end-to-end) using DNA ligases. Answer : (d)
Q.11
DNA or RNA segment tagged with a radioactive molecule is called ...[ CBSE 2010]
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a) Probe
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b) Clone
0%
c) Plasmid
0%
d) Vector
Explanation
A probe is a fragment of DNA or RNA of variable length which is used in DNA or RNA samples to detect the presence of nucleotide sequences that are complementary to the sequence in the probe. Answer : (a)
Q.12
In genetics engineering, DNA fragments are joined through ... [ ODisha 2005]
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a) Ligase
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b) Gyrase
0%
c) Helicase
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d) Polymerase
Explanation
DNA ligase is a specific type of enzyme, a ligase, that facilitates the joining of DNA strands together by catalyzing the formation of a phosphodiester bond. Answer : (a)
Q.13
Advancement in genetic engineering has been possible due to discovery of
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a) Exonucleases
0%
b) Oncogenes
0%
c) Restriction endonuclease
0%
d) Transposons
Explanation
restriction enzyme, also called restriction endonuclease, a protein produced by bacteria that cleaves DNA at specific sites along the molecule. In the bacterial cell, restriction enzymes cleave foreign DNA, thus eliminating infecting organisms. Answer : (c)
Q.14
In biochemical processes, cDNA is prepared from ... [ BHU 2012]
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a) B-DNA
0%
b) hnRNA
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c) Z-DNA
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d) mRNA
Explanation
Complementary DNA (cDNA) is a DNA copy of a messenger RNA (mRNA) molecule produced by reverse transcriptase. Answer : (d)
Q.15
Isolation of Bt gene from bacterium Bacillus thuringiensis was undertaken in year ... [ AMU 2009]
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a) 1977
0%
b) 1980
0%
c) 1997
0%
d) 1990
Explanation
Answer : (b)
Q.16
In gel electrophoresis, differential mobility of DNA depends upon ... [ MPPMT 2011]
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a) Helical nature of DNA
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b) Charge and size of DNA
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c) Hydrogen bonding between bases
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d) Double stranded nature of DNA
Explanation
The DNA fragments separate (resolve) according to their size through sieving effect provided by the agarose gel. Hence, the smaller the fragment size, the farther it moves. Answer : (b)
Q.17
GM Bt brinjal has been developed in India for ... [RPMT 2011]
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a) Enhancing shelf life
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b) Enhancing mineral content
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c) Drought resistance
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d) Insect resistance
Explanation
The Bt brinjal has been developed to give resistance against lepidopteron insects, in particular the Brinjal Fruit and Shoot Borer (Leucinodes orbonalis)(FSB) by forming pores in the digestive system. Answer : (d)
Q.18
Enzyme required for polymerase chain reaction (PCR) is ...[ JIPMER 2000]
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a) Endonuclease
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b) Taq polymerase
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c) RNA polymerase
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d) Ribonuclease
Explanation
The enzyme extends the primers using the nucleotides provided in the reaction and the genomic DNA as template. If the process of replication of DNA is repeated many times, the segment of DNA can be amplified to approximately billion times, i.e., 1 billion copies are made. Such repeated amplification is achieved by the use of a thermostable DNA polymerase (isolated from a bacterium, Thermus aquaticus), which remain active during the high temperature induced denaturation of double stranded DNA. Answer : (b)
Q.19
Agarose extracted from sea weeds finds use in ... [ CBSE 2011]
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a) PCR
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b) Gel electrophoresis
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c) Spectrophotometry
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d) Tissue culture
Explanation
The cutting of DNA by restriction endonucleases results in the fragments of DNA. These fragments can be separated by a technique known as gel electrophoresis. Since DNA fragments are negatively charged molecules they can be separated by forcing them to move towards the anode under an electric field through a medium/matrix. Nowadays the most commonly used matrix is agarose which is a natural polymer extracted from sea weeds. Answer : (b)
Q.20
In transgenics, the expression of transgene in the target tissue is known by ...[ AFMC 2008]
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a) Reporter
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b) Transgene
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c) Enhancer
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d) Promoter
Explanation
A reporter gene is a gene cloned with a DNA sequence of interest inserted into an expression vector followed by transfer to target tissues. They are used as indicators to study gene expression in target tissue for pharmaceutical and molecular biology research. Answer : (a)
Q.21
Xenograft is a graft ... [ AMU 2012]
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a) Between genetically identical individuals
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b) Between individuals of different species
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c) From one individuals to another of same species
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d) From one area to another same individual
Explanation
Xenograft: The transplant of an organ, tissue, or cells to an individual of another species. Answer : (b)
Q.22
Which one of the following is used as vector for cloning into higher organisms? [ CBSE 2010]
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a) Salmonella typhimurium?
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b) Rhizopus nigricans
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c) Retrovirus
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d) Baculovirus
Explanation
Retroviruses have also been disarmed and are now used to deliver desirable genes into animal cells. So, once a gene or a DNA fragment has been ligated into a suitable vector it is transferred into a bacterial, plant or animal host (where it multiplies). Answer : (c)
Q.23
Restriction endonucleases used widely in RDT are obtained from ...[ KCET 2006]
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a) Plasmids
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b) Bacterial cells
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c) Bacteriophages
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d) All prokaryotic cells
Explanation
The first restriction endonuclease–Hind II, whose functioning depended on a specific DNA nucleotide sequence was isolated and characterised five years later. It was found that Hind II always cut DNA molecules at a particular point by recognising a specific sequence of six base pairs. This specific base sequence is known as the recognition sequence for Hind II. Besides Hind II, today we know more than 900 restriction enzymes that have been isolated from over 230 strains of bacteria each of which recognise different recognition sequences Answer : (b)
Q.24
Restriction enzyme are used in genetic engineering because they
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a) Cut DNA at specific base sequence
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b) Can join DNA fragments
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c) Are proteolytic enzymes which degrade harmful proteins
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d) Cut DNA at variable sites
Explanation
Each restriction endonuclease functions by ‘inspecting’ the length of a DNA sequence. Once it finds its specific recognition sequence, it will bind to the DNA and cut each of the two strands of the double helix at specific points in their sugar -phosphate backbones. Each restriction endonuclease recognises a specific palindromic nucleotide sequences in the DNA. Answer : (a)
Q.25
What is true about DNA polymerase used in PCR ... [ CBSE 2012]
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a) It is used to ligate introduced DNA in recipient cells
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b) It is isolated from virus
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c) It is active at high temperature
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d) It serves as selectable marker
Explanation
Enzyme DNA polymerase extends the primers using the nucleotides provided in the reaction and the genomic DNA as template. If the process of replication of DNA is repeated many times, the segment of DNA can be amplified to approximately billion times, i.e., 1 billion copies are made. Such repeated amplification is achieved by the use of a thermostable DNA polymerase (isolated from a bacterium, Thermus aquaticus), which remain active during the high temperature induced denaturation of double stranded DNA. Answer : (c)
Q.26
Which is used in production of insulin by genetic engineering ... [ CBSE 2008]
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a) Rhizobium
0%
b) Saccharomyces
0%
c) Mycobacterium
0%
d) Escherichia
Explanation
In 1983, Eli Lilly an American company prepared two DNA sequences corresponding to A and B, chains of human insulin and introduced them in plasmids of E. coli to produce insulin chains Answer : (d)
Q.27
Gene encoding Bt protein, specific for cotton bollworm is ... [ HPPMT 2012]
0%
a) cry II Ac
0%
b) cry I Ab
0%
c) cry IAc
0%
d) cry II Abc
Explanation
cry Bt- toxin protein exists as inactive protein but once an insect ingests the inactive toxin, it is converted into an active form of toxin due to the alkaline pH of the gut, which solubulise the crystals. The activated toxin binds to the surface of midgut epithelial cells and creates pores that cause cell swelling and lysis and eventually cause death of the insect. Specific Bt toxin genes were isolated from B. thuringiensis and incolyorated into the several plants such as cotton. The toxin is coded by a gene named cry. There are a number of them, eg, the proteins encoded by the genes cry IAc and cry II Ab control the cotton bollworms, that of cry IAb control corn borers. Answer : (c)
Q.28
Advancement in genetic engineering has been possible due to discovery of
0%
a) Transposons
0%
b) Endonucleases
0%
c) Exonucleases
0%
d) Oncogenes
Explanation
restriction enzyme, also called restriction endonuclease, a protein produced by bacteria that cleaves DNA at specific sites along the molecule. In the bacterial cell, restriction enzymes cleave foreign DNA, thus eliminating infecting organisms. Answer : (b)
Q.29
Silencing of mRNA/RNA interference has been used in development of plants resistant to ... [ CBSE 2011]
0%
a) Nematodes
0%
b) Fungi
0%
c) Insects
0%
d) Viruses
Explanation
RNA interfernce (RNAi) is a novel strategy to prevent infestation of nematode. RNAi takes place in all eukaryotic organisms as a method of cellular defense. This method involves silencing of a specific mRNA due to a complementary dsRNA molecule that binds to and prevents translation of the mRNA (silencing). The source of this complementary RNA could be from an infection by viruses having RNA genomes or mobile genetic elements (transposons) that replicate via an RNA intermediate Answer : (a)
Q.30
Identify the plasmid ..[ Kerala 2010]
0%
a) EcoRI
0%
b) pBR 322
0%
c) AIUI
0%
d) Hind III
Explanation
E. coli cloning vector pBR322 shows restriction sites such as Hind III, EcoR I, BamH I, Sal I, Pvu II, Pst I, Cla I. Other options except (b) are name of restriction site whereas option (b) is vector/plasmid. Answer : (b)
Q.31
cDNA is ...[Odisha 2003 ]
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a) Circular DNA
0%
b) Coiled DNA
0%
c) Cytoplasmic DNA
0%
d) Complementary DNA
Explanation
complementary DNA is DNA synthesized from a single-stranded RNA template in a reaction catalyzed by the enzyme reverse transcriptase. cDNA is often used to clone eukaryotic genes in prokaryotes. Answer : (d)
Q.32
DNA polymerase enzyme used in PCR is isolated from ... [ HPPMT 2012]
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a) Thermus aquaticus
0%
b) E.coli
0%
c) Salmonella typhimurium
0%
d) None of the above
Explanation
Repeated amplification in PCR technology is achieved by the use of a thermostable DNA polymerase, isolated from a bacterium Thermus aquaticus. It remain active during the high temperature induced denaturation of double stranded DNA. Answer : (a)
Q.33
Which is true ...[ CBSE mains 2010]
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a) Centromere is found in animal cells which produces aster during cell division
0%
b) Insulin gene is present in every body cell
0%
c) Nucleosome is formed of nucleotides
0%
d) DNA has a core of eight histones
Explanation
'Centromere' is found in chromosomes where two chromatids are attached.Option (a) is wrong All genes are present in all cells. Option (b) is Correct nucleosome isa structural unit of a eukaryotic chromosome, Option(c) is wrong In eukaryotic organisms, the DNA is packaged with histone proteins to create a compacted structure called a nucleosome. A nucleosome consists of a molecule of DNA wrapped around a core of eight histone proteins (an octamer).Option (d) is wrong Answer : (b)
Q.34
Herbicide resistant gene is ...[ Odisha 2006]
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a) Ct
0%
b) Mt
0%
c) Bt
0%
d) GST
Explanation
Glutathione S-transferase (GST) enzymes play role in Herbicide Metabolism Answer : (d)
Q.35
Structural involved in genetic engineering is ... [ Manipal 1997]
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a) DNA polymerase I
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b) Prochromosome
0%
c) Plastid
0%
d) Restriction endonuclease
Explanation
Restriction enzymes belong to a larger class of enzymes called nucleases. These are of two kinds; exonucleases and endonucleases. Exonucleases remove nucleotides from the ends of the DNA whereas, endonucleases make cuts at specific positions within the DNA. Answer : (d)
Q.36
A genetic disorder can be cured through ...
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a) rDNA technology
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b) Embryo transfer
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c) Hybridoma technology
0%
d) Gene therapy
Explanation
Gene therapy: Here genes are inserted into a person’s cells and tissues to treat a disease. Correction of a genetic defect involves delivery of a normal gene into the individual or embryo to take over the function of and compensate for the non-functional gene. Answer : (d)
Q.37
The technique of production of monoclonal antibodies was developed by ...[ Odisha 200]
0%
a) Watson and Crick
0%
b) Milstein and Kohler
0%
c) Bentham and Hooker
0%
d) Miescher
Explanation
Milstein and Köhler described the first technique developed for stable monoclonal antibody production in 1975. This technique consists of creating a hybridoma, a stable hybrid cell capable of producing a single type of antibody against a specific epitope present in an antigen. Answer : (b)
Q.38
A technique of deliberate manipulation of gene/ transfer of gene to a different organism is ... [ JKCMEE 2007]
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a) Gene therapy
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b) Tissue culture
0%
c) Hybridoma technology
0%
d) Genetic engineering
Explanation
Genetic engineering : Techniques to alter the chemistry of genetic material (DNA and RNA), to introduce these into host organisms and thus change the phenotype of the host organism. Answer : (d)
Q.39
With the help of DNA ligase donor DNA fragment is joined. It is called ...[ Tamil Nadu 2001]
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a) Tissue culture
0%
b) Protoplasmic fusion
0%
c) Molecular cloning
0%
d) Splicing
Explanation
During splicing, introns (Non-coding regions) are removed and exons (Coding Regions) are joined together Answer : (d)
Q.40
What is the source of EcoRI ... [ AIIMS 2011]
0%
a) Escherichia coli RI
0%
b) Escherichia coli RI 13
0%
c) Escherichia coli RX 13
0%
d) Escherichia coli RY 13
Explanation
EcoRI comes from Escherichia coli RY 13. In EcoRI, the letter ‘R’ is derived from the name of strain. Roman numbers following the names indicate the order in which the enzymes were isolated from that strain of bacteria. Answer : (d)
Q.41
The enzymes which are commonly used in genetic engineering are .. [ KCET 2003] Explanation is provided, please click on
0%
a) Restriction endonuclease and polymerase
0%
b) Endonuclease and ligase
0%
c) Restriction endonuclease and ligase
0%
d) Ligase and polymerase
Explanation
Restriction Endonuclease:These are enzymes that cut DNA at specific sites. They are properly called restriction endonucleases because they cut the bonds in the middle of the polynucleotide chain. DNA Ligase:This enzyme repairs broken DNA by joining two nucleotides in a DNA strand. It is commonly used in genetic engineering to do the reverse of a restriction enzyme, i.e. to join together complementary restriction fragments. Answer : (c)
Q.42
Which is correctly matched ... [ kerala 2011]
0%
a) Agrobacterium tumefaciens - Tumor
0%
b) pBR 322 - Enzyme
0%
c) Thermus aquaticus - Bt gene
0%
d) Ligase - Molecular scissors
Explanation
When cut by the same restriction enzyme, the resultant DNA fragments have the same kind of ‘sticky-ends’ and, these can be joined together (end-to-end) using DNA ligases. Option (d) is incorrect. DNA polymerase is isolated from a bacterium, Thermus aquaticus, which remain active during the high temperature induced denaturation of double stranded DNA. Option(c) is incorrect. pBR322 is a plasmid and was one of the first widely used E. coli cloning vectors. Optin (b) is incorrect. The soil-dwelling bacterium Agrobacterium tumefaciens causes crown gall disease of dicots, which is a type of plant tumor. It does so by inserting a piece of a large plasmid it carries into susceptible plant cells. The plasmid is known as the Ti plasmid and the inserted DNA is called T-DNA Option (a) is correct. Answer : (a)
Q.43
Some of the steps involved in production of humulin are given below. Choose the correct sequence ... [ KCET 2010] i. Synthesis of insulin gene artificially ii. Culturing recombinant E. Coli in bioreactors iii. Purification of humulin iv. Insertion of human insulin gene into plasmid v. Introduction of recombinant plasmid into E.coli vi. Extraction of recombinant gene product from E.coli
0%
a) ii, i, iv, iii, v, vi
0%
b) i, iv, v, ii, vi, iii
0%
c) i, iii, v, vi, ii, iv
0%
d) iii, v, ii, i, vi, iv
Explanation
"Humulin" or Recombinant Human Insulin. The correct sequence is 1.Synthesis of the gene for human insulin artificially. 2.Insertion of human insulin in plasmid. 3.Introduction of recombinant plasmid into E.coli. 4. Culturing recombinant E.coli in bioreactors. 5.Extraction of a recombinant gene product from E coli. 6.Purification of Humulin. Answer : (b)
Q.44
Flavr Savr variety of Tomato is ..[ DPMT 2005]
0%
a) High yielding hybrid variety
0%
b) High yielding new variety
0%
c) Transgenic
0%
d) Polyploid
Explanation
Flavr Savr, a genetically modified tomato, was the first commercially grown genetically engineered food to be granted a license for human consumption. Answer : (c)
Q.45
Two bacteria most useful in genetic engineering are ...[ CBSE 1998]
0%
a) Rhizobium and Azotobacter
0%
b) Escherichia and Agrobacterium
0%
c) Rhizobium and Diplococcus
0%
d) Nitrosomonas and Klebsiella
Explanation
Two of the most important bacteria used in genetic engineering experiments are Escherichia coli (E. coli) and Agrobacterium tumefaciens. E. coli in genetic engineering include the production of human insulin. The tumor inducing (Ti) plasmid of Agrobacterium tumifaciens has now been modified into a cloning vector which is no more pathogenic to the plants but is still able to use the mechanisms to deliver genes of our interest into a variety of plants Answer : (b)
Q.46
PCR proceeds in three distinct steps governed by temperature. They are in order of ... [ DPMT 2011]
0%
a) Denaturation, synthesis, annealing
0%
b) Annealing, synthesis, denaturation
0%
c) Synthesis, annealing, denaturation
0%
d) Denaturation, annealing, synthesis
Explanation
PCR is based on three simple steps required for any DNA synthesis reaction: (1) denaturation of the template into single strands; [temperature: 94°C-95°C] (2) annealing of primers to each original strand for new strand synthesis;[Temperature:50°C-56°C] (3) extension of the new DNA strands from the primers. [temperature: 72°C] Answer : (d)
Q.47
A transgenic plant having higher storage protein is ... [ MHTCET 2008]
0%
a) Rice
0%
b) Maize
0%
c) Tomato
0%
d) Potato
Explanation
Nick named 'protato' the protein packed genetically modified (GM) potato contains 60 per cent more protein than a wild-type potato and has increased levels of several amino acids. In transgenic potato, the enhanced protein (albumin) localizes inside cytoplasm/vacuole. Answer : (d)
Q.48
Hybridoma is connected with ... [ AFMC 2011]
0%
a) Growth of cancer
0%
b) Monoclonal antibody formation
0%
c) Antibody-antigen interaction
0%
d) Activity of NK cells
Explanation
Hybridoma is a culture of hybrid cells that results from the fusion of B cells and myeloma cells. Hybridoma technology is a method for producing large numbers of identical antibodies (also called monoclonal antibodies) Answer : (b)
Q.49
Restriction enzyme EcoR I cleavages DNA at the sequence ...[ Odisha 2001]
0%
a) AAGCTT
0%
b) AAGTTC
0%
c) GTATATC
0%
d) GAATTC
Explanation
EcoRI cuts the DNA between bases G and A only when the sequence GAATTC is present in the DNA. Answer : (d)
Q.50
Which of the following has not been synthesized by DNA technology?
0%
a) Insulin
0%
b) Haemoglobin
0%
c) Somatostatin
0%
d) Interferon
Explanation
Answer : (b)
0 h : 0 m : 1 s
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