Q.1
A plant expressing a gene from another organism is ...[ HPPMT 2007]
  • a) Transgenic
  • b) Clone
  • c) Somaclonal variant
  • d) Transformed
Q.2
Bacterial plasmid contains ...[ BHU 1997]
  • a) RNA
  • b) RNA + protein
  • c) DNA
  • d) Photosynthetic structures
Q.3
Identify the vector suitable for cloning long DNA fragments ...[ EAMCET 2002]
  • a) Phage vector
  • b) Bacterial plasmid
  • c) Yeast plasmid
  • d) Cosmids
Q.4
In genetic engineering antibiotics are used ...[ CBSE 2012]
  • a) For keeping cultures free of infection
  • b) To select healthy vectors
  • c) As selectable markers
  • d) As sequences where replication starts
Q.5
In recombinant DNA technology, the term vector refers to ... [ Odisha 2010]
  • a) Plasmid that can transfer foreign DNA into a living cell
  • b) Cosmids that can cut DNA at specific base sequence
  • c) Plasmids that an join different DNA fragments
  • d) Cosmids that can degrade harmful proteins
Q.6
Assertion: Agrobacterium tumefaciens is popular in genetic engineering because this bacterium is associated with roots of all cereals and pulse crops Reason: A gene incorporated in the bacterial chromosomal genome gets automatically transferred to the crop with which the bacterium associated
  • a) Both are true with reason being correct explanation
  • b) Both are true but reason is incorrect explanation
  • c) Assertion is true but reason is wrong
  • d) Both are wrong
Q.7
Which enzyme is useful in genetic engineering?
  • a) Amylase
  • b) DNa-ase
  • c) Restriction endonuclease
  • d) Lipase
Q.8
Biopiracy is .. .. [ MHCET 2010]
  • a) Exploitation of bioresources
  • b) Patenting bioresources of others
  • c) Use of bioresources without authorisation
  • d) Both b and c
Q.9
Which is employed for synthesis of monoclonal antibody by hybridoma technique ...
  • a) RBC
  • b) Liver cells
  • c) Tumour cells
  • d) Nerve cells
Q.10
Genetically engineered microorganism used successfully in bioremediation of oil spills is ... [ MPPMT 2009]
  • a) Trichoderma
  • b) Xanthomonas
  • c) Bacillus
  • d) Pseudomonas
Q.11
Abnormal gene is replaced by normal gene. It is called ...[ DPMT 2002]
  • a) Cloning
  • b) Gene therapy
  • c) Mutation
  • d) None of the above
Q.12
Transgenic animals are those which have ...[ CET Chd 2011]
  • a) Foreign DNA in all its cells
  • b) Foreign DNA in some of its cells
  • c) DNA and RNA both in the cells
  • d) Foreign RNA in all its cells
Q.13
T1 plasmid is used for making transgenic plants. It is obtained from ...[ CBSE 2004]
  • a) Azotobacter
  • b) Agrobacterium
  • c) Rhizobium in leguminous root
  • d) Yeast
Q.14
Restriction enzyme(s) of recombinant DNA technology that make staggered cuts leaving sticky ends is/are ... [ AMU 2012]
  • a) EcoRI
  • b) Hind III
  • c) Bam HI
  • d) All the above
Q.15
Stirred-tank bioreactors have been designed for ... [ CBSE 2010]
  • a) Purification of the product
  • b) Ensuring anaerobic conditions in culture vessel
  • c) Addition of preservatives to the product
  • d) Availability of oxygen throughout the process
Q.16
Fragments of DNA formed after treatment with endonucleases are separated by technique ..[ EAMCET 2005]
  • a) Colony hybridization
  • b) Electrophoresis
  • c) Southern blotting
  • d) Polymerase chain reaction
Q.17
It is now possible to breed plants and animals of desired characters through ... [ AIIMS 1996]
  • a) Tissue culture
  • b) Genetic engineering
  • c) Ikebana technique
  • d) Chromosome engineering
Q.18
The problem of blindness in poor countries can be overcome by using ... [ CBSE 2012]
  • a) Golden Rice
  • b) Transgenic Maize
  • c) Transgenic Tomato
  • d) Bt Brinjal
Q.19
Product of biotechnology is ... [ MPPMT 2009]
  • a) Transgenic crop
  • b) Biofertilizer
  • c) Humulin
  • d) All the above
Q.20
Human proteins can be produced in milk or semen of farm animals ..[ JKCET 2012]
  • a) True
  • b) False, proteins cannot be produced in semen
  • c) False, proteins cannot be produced in milk
  • d) False, animals are not used for protein production
Q.21
Introduction of genetically modified food is not desirable because ... [ CBSE 2002]
  • a) It will affect economy of developing countries
  • b) The products are less tasty
  • c) They are costly
  • d) There is danger of entry of toxins and virus in food
Q.22
Most abundant prokaryotes helpful to humans in making curd and antibiotics are ...[ CBSE 2012]
  • a) Archaebacteria
  • b) Cyanobacteria
  • c) Heterotrophic bacteria
  • d) Chemoautotrophs
Q.23
Salt tolerant gene from Bacillus thuringiensis is introduced using T1 plasmid of ... [ AMU 2010]
  • a) Escherichia cli
  • b) Agrobacterium tumefaciens
  • c) Haemophilus influenzae
  • d) Arabidopsis thaliana
Q.24
Amplification of gene of interest by using PCR may go upto ... [ AMU 2012]
  • a) 0.1 million
  • b) 1.0 million
  • c) 1.0 billion
  • d) 1.0 trillion
Q.25
Crystals of Bt toxin produced by some bacteria do not kill the bacteria because ...[ HPPMT 2010]
  • a) Bacteria are resistant to the toxin
  • b) Toxin is inactive
  • c) Toxin is immature
  • d) Bacteria enclose toxin in a special sac
Q.26
Vitamin A rich gene is ...[ ODisha 2006]
  • a) Flavr Savr Tomato
  • b) Golden rice
  • c) Bt cotton
  • d) Vaccinated Potato
Q.27
Restriction endonucleases are ...[ CBSE 2004]
  • a) Used for vitro DNA
  • b) Present in mammalian cells for degeneration of DNA of dead cells
  • c) Used in genetic engineering for uniting two DNA molecules
  • d) Synthesised by bacteria for their defense
Q.28
Which is wrongly matched ...[ CBSE 2012]
  • a) Somatic hybridization - Fusion of two diverse cells
  • b) Vector DNA - Site for tRNA synthesis
  • c) Micropropagation - In Vitro production of plants in large number
  • d) Callus-Unorganized mass of cells produced in tissue culture
Q.29
Transgenic plants are ones ... [ CBSE 2009]
  • a) Grown on artificial medium after hybridization in the field
  • b) Produced after protoplast fusion in artificial medium
  • c) Produced by somatic embryo on artificial medium
  • d) Generated by introducing foreign DNA into cell and regenerating plant from that cell
Q.30
Which is used to select genes from genomic library ... [ JKCET 2012]
  • a) Cloning vector
  • b) DNA probes
  • c) Restriction enzyme
  • d) Gene targets
Q.31
Which is related to genetic engineering? [ AIIMS 2001]
  • a) Plastid
  • b) Plasmid
  • c) Heterosis
  • d) Mutation
Q.32
Gene amplification using primers can be done by ...[ kerala 2011]
  • a) Microinjection
  • b) ELISA
  • c) Polymerase chain reaction
  • d) Electrophoresis
Q.33
Which is used in gene cloning ... [ CBSE 2010]
  • a) Lomasomes
  • b) Mesosomes
  • c) Plasmids
  • d) Nucleotides
Q.34
Autonomously replicating circular extra chromosomal DNA is called ..[ HPPMT 2010]
  • a) Chromatin
  • b) Plasmid
  • c) Palindromic nucleotide sequence
  • d) Nucleoid
Q.35
First step in Southern blot technique is ... [ AIIMS 2008]
  • a) Digestion of DNA by restriction enzyme
  • b) Production of group of genetically identical cells
  • c) Denaturation of DNA on the gel for hybridization with specific probe
  • d) Denaturation of DNA from a nucleated cell as from the scene of crime
Q.36
Satellite DNA is useful tool in ... [ CBSE 2010]
  • a) Forensic science
  • b) Organ transplantation
  • c) Genetic engineering
  • d) Sex determination
Q.37
Genetically engineered bacteria are being used in commercial production of .. [ CBSE 1996]
  • a) Melatonin
  • b) Testosterone
  • c) Human insulin
  • d) Thyroxine
Q.38
Golden colour of Rice is due to occurrence of ... [ MHTCET 2011]
  • a) Vitamin A
  • b) Vitamin C
  • c) Vitamin B6
  • d) Vitamin K
Q.39
A correct pair of characteristics of molecular probe are ... [ EAMCET 2009] A. Very long molecule B. Double stranded C. DNA or RNA D. Complementary to a part of desired gene
  • a) A, B
  • b) B, C
  • c) C, D
  • d) D, A
Q.40
Recombinant DNA bearing ampicillin resistance gene is passed in E.coli. The latter are spread on agar plates containing ampicillin. Then ... [ AMU 2011]
  • a) Transformed recipient cells grow and untransformed cell die
  • b) Both transformed and untransformed cells die
  • c) Transformed recipient cell grow and untransformed cells die
  • d) Transformed recipient cells die and untransformed cells grow
Q.41
Genetic engineering is ... [BHU 2003]
  • a) Making artificial genes
  • b) Hybridization of DNA
  • c) Making artificial limbs and diagnostic instruments
  • d) Production of alcohol by using microorganisms
Q.42
Which one is not used for early molecular diagnosis ... [HPPMT 2011]
  • a) Polymerase chain reaction
  • b) Polyacrylamide gel electrophoresis
  • c) Recombinant DNA technology
  • d) Enzyme linked immunosorbent assay
Q.43
Transgenic plants are plants having ... [ Odisha 2002]
  • a) No gene
  • b) Genes in transposition
  • c) Genes have no function to perform
  • d) Genes of an other organism
Q.44
Globular protein of 6kDa consisting of 51 amino acids arranged in two polypeptide chains held by disulphide bridge is ... [ AMU 2012]
  • a) Keratininsulin
  • b) keratin
  • c) glucagon
  • d)fibrinogen
Q.45
A good vector in genetic engineering is ...[ JIPMER 2000]
  • a) Agrobacterium tumefaciens
  • b) Bacillus thuringiensis
  • c) Bacillus amyloliquefaciens
  • d) Salmonella typhimurium
Q.46
Assertion: A gene from Bacillus thuringiensis is incorporated in plant genome to increase yield Reason: It is Bt toxin producing gene which kills larvae of insects
  • a) Both are true with reason being correct explanation
  • b) Both are true but reason is not correct explanation
  • c) Assertion is true but reason is wrong
  • d) Both are wrong
Q.47
Polymerase chain reaction is useful in ...[ BHU 2008]
  • a) DNA synthesis
  • b) DNA amplification
  • c) Protein synthesis
  • d) Amino acid synthesis
Q.48
GMO technology is useful for ...[ HPPMT 2011]
  • a) Enhancing nutritional value of food
  • b) Helping to reduce post-harvest losses
  • c) Making crop more tolerant to abiotic stresses
  • d) All the above
Q.49
Bt cotton genes repel ...[ CET Chd. 2010]
  • a) Bacterial pathogens
  • b) Fungal pathogens
  • c) Nematode parasites
  • d) Insect pest
Q.50
Genomic DNA library is ... [ JKCMEE 2007]
  • a) Packing of donor donor DNA in a collection of vectors
  • b) A collection of gene vector
  • c) Collection of organisms for extracting DNA
  • d) A collection of literature about DNA
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