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Q.1
Genetic engineering is ...[ DPMT 1996]
0%
a) Plastic surgery
0%
b) Study of extra nuclear genes
0%
c) Addition or removal of genes
0%
d) All the above
Explanation
Answer : (c)
Q.2
Biolistic technique is used in ....[ WB 2011]
0%
a) Germplasm conservation process
0%
b) Tissue culture process
0%
c) Hybridization process
0%
d) Gene transfer process
Explanation
A gene gun is used for delivery of exogenous DNA to cells. This method is known as 'biolistics'. This device injects cells with genetic information using a heavy metal element bullet coated with plasmid DNA The gene gun method is a method used for genetically modifying plants Answer : (d)
Q.3
Transgenic hirudin is obtained from ... ... [ DPMT 2011]
0%
a) Ocimum sanctum
0%
b) Brassica napus
0%
c) Potato
0%
d) Tomato
Explanation
Answer : (b)
Q.4
Enzymes necessary for recombinant DNA technology are ... [ KCET 2012]
0%
a) Restriction endonucleases and topoisomerases
0%
b) Peptidases and ligases
0%
c) Endonucleases and polymerases
0%
d) Restriction endonucleases and ligases
Explanation
Answer : (d)
Q.5
Bacterium commonly used in plant genetic engineering is ...[ CBSE 2009]
0%
a) Corynebacterium
0%
b) Bacillus subtilis
0%
c) Agrobacterium
0%
d) Salmonella typhi
Explanation
Agrobacterium has become the major vector in plant genetic engineering. The ability of Agrobacterium to transfer genes to plants and fungi is used in biotechnology, in particular, genetic engineering for plant improvement. Answer : (c)
Q.6
There is a restriction endonuclease called EcoRI. What does 'co' part of it stands for ... [ CBSE 2011]
0%
a) Coenzyme
0%
b) coli
0%
c) Colon
0%
d) Coelom
Explanation
Answer : (b)
Q.7
Ti plasmid transforms cells of ...[ Odisha 2003]
0%
a) Animals
0%
b) Plants
0%
c) Bacteria
0%
d) Fungi
Explanation
Answer : (b)
Q.8
Saurce of Taq polymerase used in PCR is a ..[ DPMT 2010]
0%
a) Thermophilic fungi
0%
b) Halophilic bacterium
0%
c) Thermophilic bacterium
0%
d) Mesophilic fungi
Explanation
Answer : (c)
Q.9
Vector for T-DNA is ...[ Kerala 2010]
0%
a) Salmonella typhimurium
0%
b) Thermus aquaticus
0%
c) Agrobacterium tumefaciens
0%
d) Bacillus thuringiensis
Explanation
Answer : (c)
Q.10
Electroporation is ... [ AIIMS 2005]
0%
a) Making transient pores in cell membranes to introduce gene constructs
0%
b) Fast passage of nutrients through phloem sieve pores by electric stimulation
0%
c) Opening of stomata by artificial light during night
0%
d) Purification of saline water with the help of membrane system
Explanation
Answer : (a)
Q.11
Transgenic plants are developed by ... [ AIIMS 1996]
0%
a) Introducing foreign genes
0%
b) Introducing gene mutation
0%
c) Deleting certain chromosome parts
0%
d) Stopping spindle formation
Explanation
Answer : (a)
Q.12
In hybridoma technology .. [ AMU 2011]
0%
a) B-cells are fused with myeloma cells
0%
b) T-cells are fused with myeloma cells
0%
c) T-cells are fused with B-cells
0%
d) None of the above
Explanation
Answer : (a)
Q.13
Select DNA sequence which would act as a restriction site ...
0%
a) AACCGG TTGGCC
0%
b) GGTTGG CCAACC
0%
c) AAGGCT TTCCGA
0%
d) CTGCAG GACGTC
Explanation
Answer : (d)
Q.14
A patient brought to hospital with myocardial infarction is immediately given ...[ CBSE 2012]
0%
a) Statins
0%
b) Streptokinase
0%
c) Penicillin
0%
d) Cyclosporin-A
Explanation
Answer : (b)
Q.15
Thermal cycle is used in ...[ KCET 2001]
0%
a) Radio activation
0%
b) Chemical reaction
0%
c) Polymerase chain reaction
0%
d) Enzyme catalysed reaction
Explanation
Answer : (c)
Q.16
Which is correct match of phenomenon and its explanation ... [ AIIMS 2011]
0%
a) Central dogma - RNA → DNA → Protein → RNA
0%
b) Reverse transcription polymerase chain reaction (RT-PCR) - Many copies of DNA sequence
0%
c) Transcription - Formation of RNA and proteins
0%
d) RNA silencing - Use of dsRNA
Explanation
Answer : (b)
Q.17
DNA segment cleaved by EcoRI is ...[ Kerala 2003]
0%
a) ATTCGA TAAGCT
0%
b) GAATTC CTTAAG
0%
c) GCTTAA CGAATT
0%
d) GTTCAACAAGTT
Explanation
Answer : (b)
Q.18
Tumor inducing plasmid used in producing transgenic plants is that of ...[ AIIMS 2005]
0%
a) Escherichia coli
0%
b) Bacillus thuringiensis
0%
c) Agrobacterium tumefaciens
0%
d) Staphylococcus aureus
Explanation
Answer : (c)
Q.19
Genetic engineering has helped in production of ... [ AFMC 2010]
0%
a) Thyroxine
0%
b) Insulin
0%
c) Parathormone
0%
d) Epinephrine
Explanation
Answer : (b)
Q.20
DNA is generally methylated at ...[ Odisha 2005]
0%
a) A- base
0%
b) C - base
0%
c) G - base
0%
d) T- base
Explanation
In mammals, DNA methylation occurs at cytosines (C-base) in any context of the genome. Answer : (b)
Q.21
Polymerase chain reaction employs ... [ CET Chd 2009]
0%
a) Primers and DNA ligase
0%
b) DNA ligase only
0%
c) DNA polymerase only
0%
d) Primers and DNA polymerase
Explanation
Answer : (d)
Q.22
Illegal and unlawful development of biomaterial without payment to inhabitants of their origin is called ...[ JKCMEE 2010]
0%
a) Biotechnology
0%
b) Biowar
0%
c) Biopatent
0%
d) Biopiracy
Explanation
Answer : (d)
Q.23
Restriction enzymes are also called ... [ AMU 2011]
0%
a) Molecular makers
0%
b) Vectors
0%
c) Carriers
0%
d) Molecular scissors
Explanation
Answer : (d)
Q.24
Cyanogen bromide is employed in ...
0%
a) Tissue culture
0%
b) Hybridoma technology
0%
c) Synthesis of humulin
0%
d) Genetic fingerprinting
Explanation
Humulin N, NPH, human insulin (recombinant DNA origin) isophane suspension. Humulin is human insulin used for treating diabetes. In this case, genes coding for human insulin are inserted into bacteria. Bacteria produce insulin, which is harvested and used as the active ingredient in Humulin. The bacterium's DNA is then treated with cyanogen bromide, a reagent that splits protein chains at the methionine residues. This separates the insulin chains from the rest of the DNA. Answer : (c)
Q.25
Endonuclease is employed in ..[ DPMT 2002]
0%
a) Transcription
0%
b) Translation
0%
c) Genetic engineering
0%
d) DNA replication
Explanation
Answer : (c)
Q.26
Maximum number of existing transgenic animals is of ... [ CBSE 2011]
0%
a) Fish
0%
b) Pig
0%
c) Mice
0%
d) Cow
Explanation
Answer : (c)
Q.27
The most extensively used bacteria in genetic engineering is ...[ Manipal 2005]
0%
a) Bacillus
0%
b) Clostridium
0%
c) Escherichia
0%
d) Salmonella
Explanation
Answer : (c)
Q.28
First transgenic plant released for commercial use was ... [ Chd. CET 2012]
0%
a) Golden rice
0%
b) Tobacco
0%
c) Bt Cotton
0%
d) Solan gola
Explanation
Answer : (b)
Q.29
Maturation of proinsulin into insulin takes place after ...[ AMU 2011]
0%
a) Joining of c-peptide
0%
b) Removal of c-peptide
0%
c) Removal of disulphide bridge
0%
d) Addition of disulphide bridge
Explanation
In mammals, including humans, insulin is synthesised as a pro-hormone (like a pro-enzyme, the pro-hormone also needs to be processed before it becomes a fully mature and functional hormone) which contains an extra stretch called the C peptide. This C peptide is not present in the mature insulin and is removed during maturation into insulin Answer : (b)
Q.30
Match the columns and find the correct combination
I
II
a)
Bacillus thuringiensis
Production of chitinases
b)
Rhizobium melitoti
Scavenging of oil spills
c)
Escherichia coli
Incorporation of
nif
gene
d)
Pseudomonas putida
Production of Bt toxin
e)
Trichoderma
Production of human insulin
0%
a) a- 2, b-4, c- 1, d - 5, e - 3
0%
b) a- 2, b-4, c- 5, d - 1 e - 3
0%
c) a- 4, b-3, c- 5, d - 2, e - 1
0%
d) a- 4, b-2, c- 5, d - 3, e - 1
Explanation
Answer : (c)
Q.31
The technique of insertion of a desired gene into DNA of plasmid vector is ...[ KCET 1999]
0%
a) Gene splicing
0%
b) Gene dressing
0%
c) Gene cloning
0%
d) Gene drafting
Explanation
Gene splicing technology, allows researchers to insert new genes into the existing genetic material of an organisms genome so that entire traits, from disease resistance to vitamins, and can be copied from one organism and transferred another. Gene cloning is the process in which a gene of interest is located and copied (cloned) out of DNA extracted from an organism. Genetic hitchhiking, also called genetic draft or the hitchhiking effect, is when an allele changes frequency not because it itself is under natural selection, but because it is near another gene that is undergoing a selective sweep and that is on the same DNA chain. Answer : (a)
Q.32
Clot buster obtained from Streptococcus and modified by genetic engineering is ...[ Kerala 2012]
0%
a) Streptokinase
0%
b) Penicillin
0%
c) Cyclosporin A
0%
d) Statins
Explanation
Answer : (a)
Q.33
Which of these is used as vector for gene therapy in SCID and gene cloning in higher organisms? ... ..[ RPPMT 2011]
0%
a) Retrovirus
0%
b) Enterovirus
0%
c) Arbovirus
0%
d) Rotavirus
Explanation
Answer : (a)
Q.34
Main aim of human genome project is to ... [ JKCMEE 2010]
0%
a) Develop better techniques to compare two human DNAs
0%
b) Identify and sequence all the genes present in human DNA
0%
c) Remove disease causing genes from human DNA
0%
d) Introduce new genes into human into human beings
Explanation
Answer : (b)
Q.35
Some of the characteristics of Bt cotton are ... [ CBSE 2010]
0%
a) Medium yield, long fibre and resistance to beetle pests
0%
b) High yield and production of toxic proteins crystals which kill dipteran pests
0%
c) High yield and resistance to bollworms
0%
d) Long fibre and resistance to aphids
Explanation
Answer : (c)
Q.36
Which is correctly matched ...[ BHU 2008]
0%
a) Centrak dogma - codon
0%
b) RNA polymerase - RNA primer
0%
c) Okazaki fragments - splicing
0%
d) Restriction enzyme - genetic engineering
Explanation
Answer : (d)
Q.37
Which of the following is a transgenic plant ...[ Odisha 2010]
0%
a) Flavr Savr
0%
b) Ashbya gossypii
0%
c) Meloidogyne icognita
0%
d) Gluconobacter oxydans
Explanation
Answer : (a)
Q.38
Micro-particles for coating with DNA to be bombarded with gene gun are made of ...[ CBSE 2012]
0%
a) Gold or tungsten
0%
b) Platinum or zinc
0%
c) Silver or platinum
0%
d) Silicon or platinum
Explanation
Answer : (a)
Q.39
Which can be used as vector for transfer of DNA segment ... [CBSE 2010] A) Bacterium B) Plasmid C) Plasmodium D) Bacteriophage
0%
a) A, B and C
0%
b) A only
0%
c) A and C
0%
d) B and D
Explanation
Answer : (d)
Q.40
Production of human protein in bacteria by genetic engineering is possible because ... [ CBSE 2005]
0%
a) Human chromosomes replicate in bacterial cell
0%
b) Mechanism of gene regulation is identical in human and bacteria
0%
c) Bacterial cell can undertake RNA splicing
0%
d) Genetic code is universal
Explanation
Answer : (d)
Q.41
Restriction endonuclease is employed for cutting ... [ BHU 1995]
0%
a) A single strand DNA
0%
b) Double strand DNA
0%
c) RNA fragment
0%
d) mRNA
Explanation
Answer : (b)
Q.42
"World Intellectual Property Day" is observed on
0%
a) February 29th
0%
b) April 26th
0%
c) September 5th
0%
d) June 30th
Explanation
Answer : (b)
Q.43
Which is used in recombinant DNA technology? [ Guj CET 2009]
0%
a) Virus
0%
b) Capsid of virus
0%
c) Cell wall of virus
0%
d) Gene which produces capsid of virus
Explanation
Answer : (d)
Q.44
Flavr Savr Tomato has increased ...[ MHT CET 2011]
0%
a) Productivity
0%
b) Vigour
0%
c) Shelf life
0%
d) Flowering period
Explanation
The benefit of the Flavr Savr tomato was its resistance to rotting. This was achieved by modifying the tomato with a gene that blocked the production of an enzyme that caused the tomato to rot. This enzyme was responsible for the softening of the tomato's cell wall during ripening. Answer : (c)
Q.45
Restriction endonuclease is used in ... [ MPPMT 1999 ]
0%
a) Tissue culture
0%
b) Genetic engineering
0%
c) Cell fractionation
0%
d) Regeneration
Explanation
Answer : (b)
Q.46
A regulatory body working under MoEF for the release of transgenic crop is ... [ DPMT 2011]
0%
a) NBPGR
0%
b) NSC
0%
c) NIPGR
0%
d) GEAC
Explanation
Answer : (d)
Q.47
Construction of recombinant DNA involves ... [ KCET 2002]
0%
a) Cleaving and rejoining of DNA segments with endonuclease
0%
b) Cleaving DNA segments with endonuclease and rejoining with ligase
0%
c) Cleaving and rejoining DNA segments with ligase
0%
d) Cleaving DNA segments with ligase and rejoining with endonuclease
Explanation
Answer : (b)
Q.48
Which is not a pharmaceutical product obtained through biotechnology
0%
a) Cholecystokinin
0%
b) Clotting factor
0%
c) Human growth hormone
0%
d) Human insulin
Explanation
Answer : (a)
Q.49
Which is obtained from genetic engineering .... [ DPMT 2008]
0%
a) Glucose
0%
b) Haemoglobin
0%
c) Golden Rice
0%
d) None of the above
Explanation
Answer : (c)
Q.50
Bt gene is got from ...[ HPPMT 2007]
0%
a) Brassica napus
0%
b) Bacillus thuringiensis
0%
c) Azolla
0%
d) Rhizobium
Explanation
Answer : (b)
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