Q.1
Only one cycle of directed evolution cycle is sufficient to obtain the desired protein molecule.
  • a) True
  • b) False
Q.2
The starting point of both rational and irrational protein design is a wild-type protein.
  • a) False
  • b) True
Q.3
Which step is performed after the mutation of genes in directed evolution?
  • a) X-ray crystallography
  • b) Protein assay
  • c) Protein screening
  • d) Expression of mutant genes
Q.4
Which of the following is not a method of directed evolution?
  • a) Phage display
  • b) DNA shuffling
  • c) Error-prone PCR
  • d) Natural selection
Q.5
Who pioneered phage-display technology?
  • a) Kary Mullis
  • b) Charles Chamberland
  • c) Robert brown
  • d) George Smith
Q.6
In the case of monovalent display, the valency of display depends on the ratio of phage containing phagemid vector and helper phage at the time of infection.
  • a) False
  • b) True
Q.7
Which of the following bacteriophage is used in phage-display technology?
  • a) T4
  • b) λ
  • c) T2
  • d) M13
Q.8
Mis a filamentous phage and it contains a circular single-stranded DNA genome.
  • a) False
  • b) True
Q.9
Which of the following is the major coat protein of M13?
  • a) gp VI
  • b) gp V
  • c) gp IV
  • d) gp VIII
Q.10
Which of the following is not true for error-prone PCR?
  • a) It uses Taq polymerase which lacks proofreading ability
  • b) Occurs under imperfect PCR conditions
  • c) Involves annealing incompatible base-pairs to each other
  • d) It is not a mutagenesis method
Q.11
Which of the following proteins are involved in initiating phage assembly and maintaining the stability of the viral particle?
  • a) gp III and gp VIII
  • b) gp VIII and gp II
  • c) gp VI and gp IV
  • d) gp VII and gp IX
Q.12
In the case of a monovalent display, the protein fusion can be constructed in a phagemid vector that carries a copy of the viral capsid gene.
  • a) False
  • b) True
Q.13
Which of the following is used to create a large library of mutants for a given protein gene?
  • a) Protein assay
  • b) Phage display
  • c) X-ray crystallography
  • d) Error-prone PCR
Q.14
In the case of polyvalent-display, all gp III are conjugated with peptide and this results in occasional loss of infectivity.
  • a) False
  • b) True
Q.15
In the case of a polyvalent display, the proteins can be cloned directly into a viral vector as a fusion to a capsid protein.
  • a) False
  • b) True
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