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Protein Engineering
Protein Engineering Assessment
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Q.1
Cellulose is a hybrid enzyme.
a) False
b) True
Q.2
Which of the following is the final, but an essential component of rational design?
a) X-ray crystallography
b) Computer modeling
c) Protein assay
d) Site-directed mutagenesis
Q.3
Which step is performed after the variant protein is expressed and purified?
a) Protein manipulation
b) Computer-aided modeling
c) Site-directed mutagenesis
d) Evaluation of desired property
Q.4
Rational protein design is a high throughput protein engineering.
a) True
b) False
Q.5
X-ray crystallography is not required in each and every cycle of rational protein design.
a) True
b) False
Q.6
Only one cycle of rational protein design is sufficient to get a protein with desired characteristics.
a) True
b) False
Q.7
In the rational design of proteins, once variant proteins are made and purified the new protein is evaluated to see if the desired property is achieved.
a) False
b) True
Q.8
What is the missing component in the flow diagram of the rational design of proteins given below?
a) Protein-ligand assay
b) Structure visualization
c) Structure prediction
d) Site-directed mutagenesis
Q.9
In rational design, knowledge of the structure and function of the protein is taken into consideration.
a) False
b) True
Q.10
Which of the following is done for the directed evolution of the proteins with an unknown structure?
a) Site-directed mutagenesis
b) Specific mutations
c) Non-random mutations
d) Random changes (mutations)
Q.11
Subtilisin (a protease) is inactivated by bleach due to the presence of which amino acid?
a) Cysteine at 23
b) Serine at 32
c) Alanine 33
d) Methionine at 22
Q.12
Which of the following mutation in subtilisin makes it resistant to inactivation by bleach?
a) Serine 32 → glycine
b) Alanine 33 → methionine
c) Cysteine 23 → valine
d) Methionine 22 → alanine
Q.13
Which of the following is a strategy in protein engineering attempts to create improved protein molecules based on the three-dimensional structure and the relationship between structure and function?
a) Natural evolution
b) Directed evolution
c) Irrational design
d) Rational design
Q.14
Which of the following process involves the determination of the three-dimensional structure of a protein and representing it graphically and mathematically on a computer?
a) Irrational design
b) Directed evolution
c) Natural evolution
d) Rational design
Q.15
Which of the following step in rational design allows predictions to be made particularly in the realm of the effect of mutations on structure-based properties?
a) Site-directed mutagenesis
b) X-ray crystallography
c) Protein assay
d) Computer modeling
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