Q.1
Cellulose is a hybrid enzyme.
  • a) False
  • b) True
Q.2
Which of the following is the final, but an essential component of rational design?
  • a) X-ray crystallography
  • b) Computer modeling
  • c) Protein assay
  • d) Site-directed mutagenesis
Q.3
Which step is performed after the variant protein is expressed and purified?
  • a) Protein manipulation
  • b) Computer-aided modeling
  • c) Site-directed mutagenesis
  • d) Evaluation of desired property
Q.4
Rational protein design is a high throughput protein engineering.
  • a) True
  • b) False
Q.5
X-ray crystallography is not required in each and every cycle of rational protein design.
  • a) True
  • b) False
Q.6
Only one cycle of rational protein design is sufficient to get a protein with desired characteristics.
  • a) True
  • b) False
Q.7
In the rational design of proteins, once variant proteins are made and purified the new protein is evaluated to see if the desired property is achieved.
  • a) False
  • b) True
Q.8
What is the missing component in the flow diagram of the rational design of proteins given below?
  • a) Protein-ligand assay
  • b) Structure visualization
  • c) Structure prediction
  • d) Site-directed mutagenesis
Q.9
In rational design, knowledge of the structure and function of the protein is taken into consideration.
  • a) False
  • b) True
Q.10
Which of the following is done for the directed evolution of the proteins with an unknown structure?
  • a) Site-directed mutagenesis
  • b) Specific mutations
  • c) Non-random mutations
  • d) Random changes (mutations)
Q.11
Subtilisin (a protease) is inactivated by bleach due to the presence of which amino acid?
  • a) Cysteine at 23
  • b) Serine at 32
  • c) Alanine 33
  • d) Methionine at 22
Q.12
Which of the following mutation in subtilisin makes it resistant to inactivation by bleach?
  • a) Serine 32 → glycine
  • b) Alanine 33 → methionine
  • c) Cysteine 23 → valine
  • d) Methionine 22 → alanine
Q.13
Which of the following is a strategy in protein engineering attempts to create improved protein molecules based on the three-dimensional structure and the relationship between structure and function?
  • a) Natural evolution
  • b) Directed evolution
  • c) Irrational design
  • d) Rational design
Q.14
Which of the following process involves the determination of the three-dimensional structure of a protein and representing it graphically and mathematically on a computer?
  • a) Irrational design
  • b) Directed evolution
  • c) Natural evolution
  • d) Rational design
Q.15
Which of the following step in rational design allows predictions to be made particularly in the realm of the effect of mutations on structure-based properties?
  • a) Site-directed mutagenesis
  • b) X-ray crystallography
  • c) Protein assay
  • d) Computer modeling
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