Q.1
Which of the following is an 8-residue minimal peptide sequence that exhibits intrinsic affinity towards streptavidin and can be fused to recombinant proteins in various combinations?
  • a) Strep-tag I
  • b) S-tag
  • c) Flag peptide
  • d) Strep-tag II
Q.2
Strep-tag II hampers protein folding or secretion and it usually interferes with protein function.
  • a) True
  • b) False
Q.3
Which of the following is a homo-tetramer ofamino acids per subunit?
  • a) Biotin
  • b) Flag peptide
  • c) S-tag
  • d) Streptavidin
Q.4
Strep-tag II is sensitive to cellular proteases.
  • a) True
  • b) False
Q.5
Streptavidin is naturally secreted by which of the following microorganisms?
  • a) Aspergillus niger
  • b) Escherichia coli
  • c) Bacillus anthrax
  • d) Streptomyces avidinii
Q.6
An ideal affinity tag should interfere with the folding or bioactivity of a recombinant protein.
  • a) True
  • b) False
Q.7
Which of the following affinity tag is shown in the figure below?
  • a) Strep-tag I
  • b) Flag peptide
  • c) Myc tag
  • d) Strep-tag II
Q.8
The affinity of Strep-tag for streptavidin is very high when it is placed at the amino terminus or in between two protein domains.
  • a) True
  • b) False
Q.9
Which of the following fits in the empty box in the figure below?
  • a) Application of NaOH
  • b) Application of Biotin
  • c) Application of Strep-Tactin
  • d) Application of HABA
Q.10
Which of the following is used in the column regeneration step of Strep-tag purification, that displaces desthiobiotin?
  • a) Buffer
  • b) Biotin
  • c) NaOH
  • d) HABA
Q.11
Which of the following is an engineered streptavidin variant?
  • a) Biotin
  • b) Strep-tag II
  • c) Strep-tag I
  • d) Strep-Tactin
Q.12
Which of the following shows the highest binding affinity for Strep-Tactin?
  • a) Biotin
  • b) Strep-tag I
  • c) Strep-tag II
  • d) Twin-strep-tag
Q.13
Which of the following is used in the column regeneration step of Twin-Strep-tag: Strep-Tactin XT purification, that displaces biotin?
  • a) Desthiobiotin
  • b) Buffer
  • c) HABA
  • d) NaOH
Q.14
Strep-tag I can be used at which of the following positions?
  • a) Carboxy or amino-terminal
  • b) Amino-terminal
  • c) In between two protein domains
  • d) Carboxy terminal
Q.15
An ideal affinity tag should display an easily controllable binding behavior with sufficient affinity.
  • a) False
  • b) True
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