Q.1
Annealing is the second step in PCR. This is when:
  • DNA is copied
  • DNA strands separate
  • Primers bind to DNA
  • The PCR is finished
Q.2
What is the function of a primer?
  • To identify the particular region of DNA to be copied by PCR.
  • To copy DNA.
  • To create DNA nucleotides.
  • To maintain the temperature of the PCR reaction.
Q.3
With regards to DNA replication along the template strand, what event takes place first?
  • DNA polymerase begins adding RNA nucleotides
  • DNA polymerase begins adding DNA nucleotides
  • DNA ligase joins the Okazaki fragments
  • primase adds an RNA primer
Q.4
All of the following are thermostable polymerases except
  • Taq Polymerase
  • Vent Polymerase
  • DNA Polymerase III
  • pfu Polymerase
Q.5
You decide to use PCR to determine if you have Cystic Fibrosis, a genetic disorder. You then decide to also check if you are at risk for developing Alzheimers, another genetic disorder.What PCR "ingredient" would be different in these two tests?
  • Template DNA
  • Nucleotides
  • Taq DNA polymerase
  • Primers
Q.6
Thermus aquaticus is the source of
  • Primase enzyme
  • Taq Polymerase
  • Vent Polymerase
  • Both 2 and 3
Q.7
Which of the following statements about DNA replication is TRUE?
  • the leading strand is replicated continuously, while the lagging strand is replicated discontinuously
  • the leading strand is replicated discontinuously, while the lagging strand is replicated continuously
  • both the leading and lagging strands are replicated continuously
  • both the leading and lagging strands are replicated discontinuously
Q.8
During PCR, what is the purpose of increasing temperature to°\degree° C?
  • For primers to anneal to DNA template strands
  • To separate DNA strands
  • To denature Taq polymerase
  • For Taq polymerase to form new copies of DNA
Q.9
Which of the following is true regarding PCR
  • Denaturation involves heating at 90-98 degree C
  • Annealing involves binding of primer between 40-60 degree C
  • Primer extension occurs at 72 degree C
  • All of these
Q.10
The PCR technique was developed by
  • Karry Mullis
  • Kohler
  • Milstein
  • Altman
Q.11
PCR is a
  • DNA Sequencing technique
  • DNA Amplification technique
  • DNA Degradation technique
  • All of these
Q.12
The basic requirements of PCR reaction include
  • DNA segment to be amplified
  • Two oligonucleotide primers
  • a heat stable DNA polymerase
  • all of these
Q.13
The first step in PCR is
  • Primer annealing
  • Denaturation
  • Primer extension
  • None of these
Q.14
Denaturation involves
  • heating between 40-60 C
  • heating between 90-98 C
  • heating between 70-72 C
  • None of these
Q.15
Which of the following statements are true regarding PCR
  • Automated PCR machines are called thermal cyclers
  • A thermostable DNA Polymerase is required
  • Billion copies of desired DNA can be synthesized from microgram quantities of DNA
  • All of these
Q.16
Reverse transcription PCR uses
  • mRNA as a template to form
  • RNA as a template to form a DNA
  • DNA as a template to form ssDNA
  • all of these
Q.17
If two double-stranded DNA molecules are used at the beginning of a polymerase chain reaction (PCR) process, how many double-stranded DNA molecules can be obtained after two cycles?
  • 2
  • 4
  • 8
  • 32
Q.18
How is Fast PCR able to run at such a shorter time frame
  • Lower the temperature of Denaturation
  • raises the temperature for annealing so that it and extension can happen almost simultaneously
  • Shortens the time frame of denatureation
  • all of the answers are correct
Q.19
What is the function of DNA Polymerase?
  • attach primers to target sequence
  • separate the DNA
  • attach nucleotides to the target sequence
  • separate the fragments according to size
Q.20
If the primer sequence is TCG, what section of DNA will it anneal?
  • AGC
  • CAT
  • GAC
  • ATC
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