Q.1
What is the purpose of the Extension step of PCR?
  • To create the primers.
  • To extend the time it takes to produce DNA.
  • To allow polymerase to create the complementary strands of DNA.
  • To allow substrates to copy DNA sequences.
Q.2
How many cycles of PCR are normally used.
  • 20-35
  • 6
  • 45
  • 100
Q.3
What happens in the Denature step of PCR?
  • The DNA nucleotides are broken apart.
  • The base-pairing rules for DNA are reversed.
  • The double-stranded DNA is separated into two single strands of DNA.
  • The DNA is returned to its natural setting.
Q.4
PCR happens in 3 steps (denaturing, annealing, extending) or 1 "cycle". How many times are cycles typically repeated?
  • 1
  • 10
  • 30
  • Over 1 billion
Q.5
What are the base-pairing rules in DNA?
  • A-C, G-T
  • A-U, G-C
  • A-T, G-C
  • A-C, G-U
Q.6
How many cycles of PCR does it take to produce a double-stranded DNA fragment of the desired length?
  • 1
  • 2
  • 3
  • 4
Q.7
Why does PCR require a specific type of polymerase?
  • Taq polymerase can withstand the range of temperatures in PCR without denaturing.
  • Taq polymerase is the most inexpensive type of polymerase.
  • Taq polymerase is the most common type of polymerase.
  • A specific type of polymerase is unnecessary.
Q.8
At what temperature does the Extension step of PCR occur?
  • 94-96°C
  • 50-65°C
  • 72°C
  • Any temperature
Q.9
What does PCR stand for?
  • Polymerase Chronic Reagent
  • Principle Chorionic Reliability
  • Polymerase Chain Reaction
  • Probably Cannot React
Q.10
During PCR, what is the purpose of increasing temperature to$\degree$ C?
  • For primers to anneal to DNA template strands
  • To separate DNA strands
  • To denature Taq polymerase
  • For Taq polymerase to form new copies of DNA
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