Q.1
The promoters of B. subtilis vectors have conserved polyA and ________ tails.
  • a) PolyT
  • b) PolyC
  • c) PolyG
  • d) PolyA
Q.2
Ribosomes from _________ recognize only homologous mRNA.
  • a) E. coli
  • b) B. subtilis
  • c) Yeast
  • d) Bacteriocins
Q.3
The selectivity of ribosomes of B. subtilis is due to the lack of ________
  • a) Inducer
  • b) Promoter
  • c) Protein S1
  • d) Repressor
Q.4
Which of the following does not lack MRNA selectivity?
  • a) Streptococcus
  • b) Clostridium
  • c) E. coli
  • d) Lactobacillus
Q.5
The role of S1 is to bind ________ non-specifically.
  • a) MRNA
  • b) RNA
  • c) DNA
  • d) CDNA
Q.6
When was the Spac system for low-GC hosts developed?
  • a) 1990
  • b) 1984
  • c) 1963
  • d) 1936
Q.7
Which gene of E. coli is contained in the Spac system?
  • a) Tol
  • b) Amp
  • c) LacI
  • d) Gal
Q.8
A promoter from which phage is incorporated in the Spac system?
  • a) Lambda
  • b) M13
  • c) Prophage
  • d) SPO-1
Q.9
E. coli T7 system is successfully implemented in B. subtilis.
  • a) True
  • b) False
Q.10
The promoter used in T7 system is inducible by which of the following?
  • a) Xylose
  • b) Galactose
  • c) Lactose
  • d) Fructose
Q.11
Expression of a heterologous gene in the T7 system is ____________
  • a) Easy
  • b) Difficult
  • c) Not possible
  • d) Costly
Q.12
Other than B. subtilis, the T7 expression system can also be used in __________
  • a) Lactobacillus
  • b) Yeast
  • c) Fungi
  • d) Agrobacterium
Q.13
In the phi-system of L. lactis, _________ vector is used.
  • a) Hybrid
  • b) Mammalian
  • c) Low copy number
  • d) High copy number
Q.14
In the phi-system of L. lactis, gene of interest is placed under the control of _____________ promoter.
  • a) Weak
  • b) Strong
  • c) End
  • d) Middle
Q.15
Following infection with phi-__________ increases.
  • a) Copy number
  • b) Mortality rate
  • c) Size
  • d) Protein production
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