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Vector Biology Gene Manipulation
Advanced Vector Biology
Quiz 1
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Q.1
The promoters of B. subtilis vectors have conserved polyA and ________ tails.
a) PolyT
b) PolyC
c) PolyG
d) PolyA
Q.2
Ribosomes from _________ recognize only homologous mRNA.
a) E. coli
b) B. subtilis
c) Yeast
d) Bacteriocins
Q.3
The selectivity of ribosomes of B. subtilis is due to the lack of ________
a) Inducer
b) Promoter
c) Protein S1
d) Repressor
Q.4
Which of the following does not lack MRNA selectivity?
a) Streptococcus
b) Clostridium
c) E. coli
d) Lactobacillus
Q.5
The role of S1 is to bind ________ non-specifically.
a) MRNA
b) RNA
c) DNA
d) CDNA
Q.6
When was the Spac system for low-GC hosts developed?
a) 1990
b) 1984
c) 1963
d) 1936
Q.7
Which gene of E. coli is contained in the Spac system?
a) Tol
b) Amp
c) LacI
d) Gal
Q.8
A promoter from which phage is incorporated in the Spac system?
a) Lambda
b) M13
c) Prophage
d) SPO-1
Q.9
E. coli T7 system is successfully implemented in B. subtilis.
a) True
b) False
Q.10
The promoter used in T7 system is inducible by which of the following?
a) Xylose
b) Galactose
c) Lactose
d) Fructose
Q.11
Expression of a heterologous gene in the T7 system is ____________
a) Easy
b) Difficult
c) Not possible
d) Costly
Q.12
Other than B. subtilis, the T7 expression system can also be used in __________
a) Lactobacillus
b) Yeast
c) Fungi
d) Agrobacterium
Q.13
In the phi-system of L. lactis, _________ vector is used.
a) Hybrid
b) Mammalian
c) Low copy number
d) High copy number
Q.14
In the phi-system of L. lactis, gene of interest is placed under the control of _____________ promoter.
a) Weak
b) Strong
c) End
d) Middle
Q.15
Following infection with phi-__________ increases.
a) Copy number
b) Mortality rate
c) Size
d) Protein production
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