Q.1
What are the lectins?
  • a) Antigen
  • b) Antibody
  • c) DNA
  • d) RNA
Q.2
Northern Blotting is not a variant of Southern blotting.
  • a) True
  • b) False
Q.3
Which of the following is found in Northern blotting but not in southern blotting?
  • a) Gel
  • b) Nitrocellulose
  • c) Reactive paper
  • d) Probe
Q.4
The reactive paper used is prepared by which technique?
  • a) Heating
  • b) Hydrolysis
  • c) Acidification
  • d) Diazotization
Q.5
How can hybridizing bands be located?
  • a) Radiography
  • b) Autoradiography
  • c) UV radiation
  • d) Infrared radiation
Q.6
When was it found that RNA can be bound to nitrocellulose membranes under appropriate conditions?
  • a) 1980
  • b) 1880
  • c) 1940
  • d) 1950
Q.7
Nylon membranes have superseded the need of ___________ in southern blotting techniques.
  • a) Probe
  • b) RNA
  • c) DBM paper
  • d) Gel
Q.8
Which of the following techniques does not involve nucleic acids?
  • a) Northern blotting
  • b) Western blotting
  • c) PFGE
  • d) Southern blotting
Q.9
Western Blotting is used to transfer __________
  • a) Genes
  • b) Proteins
  • c) DNA
  • d) Probe
Q.10
When was the Western blotting technique developed?
  • a) 1980
  • b) 1961
  • c) 1981
  • d) 1971
Q.11
The protein bands transferred by the western blotting are previously ______________
  • a) Electrophoresed
  • b) Heated
  • c) Calibrated
  • d) Mixed
Q.12
In Western blotting, proteins bind __________ to the membrane.
  • a) Loosely
  • b) Covalently
  • c) Irreversibly
  • d) Strongly
Q.13
Which interactions are made use of in Western blotting technique?
  • a) Covalent
  • b) Hydrophobic
  • c) Protein-ligand
  • d) Protein-protein
Q.14
Which is the most common ligand in Western blotting?
  • a) Lactose
  • b) Toxins
  • c) Genes
  • d) Antibodies
Q.15
Lectins identify which of the following in a Western blotting technique?
  • a) Glucoprotein
  • b) Glycoprotein
  • c) Antibiotic
  • d) RNA
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