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Vector Biology Gene Manipulation
Gene Manipulation Written Test
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Q.1
What are the lectins?
a) Antigen
b) Antibody
c) DNA
d) RNA
Q.2
Northern Blotting is not a variant of Southern blotting.
a) True
b) False
Q.3
Which of the following is found in Northern blotting but not in southern blotting?
a) Gel
b) Nitrocellulose
c) Reactive paper
d) Probe
Q.4
The reactive paper used is prepared by which technique?
a) Heating
b) Hydrolysis
c) Acidification
d) Diazotization
Q.5
How can hybridizing bands be located?
a) Radiography
b) Autoradiography
c) UV radiation
d) Infrared radiation
Q.6
When was it found that RNA can be bound to nitrocellulose membranes under appropriate conditions?
a) 1980
b) 1880
c) 1940
d) 1950
Q.7
Nylon membranes have superseded the need of ___________ in southern blotting techniques.
a) Probe
b) RNA
c) DBM paper
d) Gel
Q.8
Which of the following techniques does not involve nucleic acids?
a) Northern blotting
b) Western blotting
c) PFGE
d) Southern blotting
Q.9
Western Blotting is used to transfer __________
a) Genes
b) Proteins
c) DNA
d) Probe
Q.10
When was the Western blotting technique developed?
a) 1980
b) 1961
c) 1981
d) 1971
Q.11
The protein bands transferred by the western blotting are previously ______________
a) Electrophoresed
b) Heated
c) Calibrated
d) Mixed
Q.12
In Western blotting, proteins bind __________ to the membrane.
a) Loosely
b) Covalently
c) Irreversibly
d) Strongly
Q.13
Which interactions are made use of in Western blotting technique?
a) Covalent
b) Hydrophobic
c) Protein-ligand
d) Protein-protein
Q.14
Which is the most common ligand in Western blotting?
a) Lactose
b) Toxins
c) Genes
d) Antibodies
Q.15
Lectins identify which of the following in a Western blotting technique?
a) Glucoprotein
b) Glycoprotein
c) Antibiotic
d) RNA
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