Q.1
The lambda P L promoter is a weak promoter.
  • a) True
  • b) False
Q.2
Lambda vector subverts which important enzyme of E.coli?
  • a) DNA polymerase
  • b) Helicase
  • c) RNA polymerase
  • d) Nuclease
Q.3
The lambda promoter is repressed by which of the following gene?
  • a) T gene
  • b) cI gene
  • c) Amp gene
  • d) Trp gene
Q.4
Expression vectors that carry the lambda P L promoter are used with ________ E.coli strain.
  • a) Double
  • b) Hybrid
  • c) Mutant
  • d) Wild-type
Q.5
Below what temperature can the cI protein repress the lambda promoter?
  • a) 10 degrees
  • b) 20 degrees
  • c) 30 degrees
  • d) 40 degrees
Q.6
At higher temperature the cI protein is __________
  • a) Inactivated
  • b) Increased
  • c) Decreased
  • d) Moderated
Q.7
The T7 promoter is specific for the __________
  • a) Mutant strains
  • b) Bacteriophage
  • c) RNA polymerase
  • d) DNA polymerase
Q.8
Gene expressed downstream of T7 promoter will be expressed at _____________
  • a) Low level
  • b) High level
  • c) Moderate level
  • d) Intermittently
Q.9
Which of the following is present in the lysogenic special strain of E.coli?
  • a) Trp promoter
  • b) Lac promoter
  • c) Ampicillin
  • d) IPTG
Q.10
How can the synthesis of T7 RNA polymerase can be switched on in a culture?
  • a) IPTG addition
  • b) IPTG removal
  • c) Ampicillin addition
  • d) Ampicillin removal
Q.11
The cassette in an expression vector comprises of ribosome binding site and ____________
  • a) Promoter
  • b) Terminator
  • c) Repressor
  • d) Inducer
Q.12
Insertion of the foreign gene into the expression vector’s cassette fuses ____ reading frames.
  • a) 1
  • b) 2
  • c) 3
  • d) 4
Q.13
Which type of protein is produced with cassette vectors?
  • a) Plant protein
  • b) Mammalian protein
  • c) Fusion protein
  • d) Hybrid protein
Q.14
P-terminus of the fusion protein is fused with a short peptide.
  • a) True
  • b) False
Q.15
Which of the following is responsible for the mRNA translation effectively?
  • a) Ribosome binding site
  • b) IPTG
  • c) Ubiquitin
  • d) Ampicillin
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