Q.1
What is the reason for a large genome of poxyvirus?
  • a) Encodes for all human genes
  • b) Encodes for replication machinery
  • c) Encodes all promoters
  • d) Contains a large portion of junk DNA
Q.2
Why are recombinant genomes of poxyvirus non-infectious?
  • a) Virus packages its own DNA
  • b) Host packages viral DNA
  • c) DNA remains unpacked
  • d) Virus packages unpacked
Q.3
5-bromodeoxyuridine is an analog of ___________
  • a) Pyrimidine
  • b) Alanine
  • c) Valine
  • d) Thymidine
Q.4
When transgene is inserted into the viral hemagglutinin locus, wild-type plaques turn ________
  • a) Red
  • b) White
  • c) Blue
  • d) Clear
Q.5
NEO is a type of ____________
  • a) Selectable marker
  • b) Screenable marker
  • c) Promoter
  • d) Inducer
Q.6
LacZ’ is a screenable marker used with Vaccinia vectors.
  • a) True
  • b) False
Q.7
Transgene expression in vaccinia vectors depends on ___________
  • a) Exogenous promoter
  • b) Endogenous promoter
  • c) Endogenous inducer
  • d) Exogenous inducer
Q.8
Highest expression levels in Vaccinia vectors are produced by ______ promoters.
  • a) P11
  • b) P7.5
  • c) 4b
  • d) P10
Q.9
Vaccinia vectors cannot be used to express genes with __________
  • a) Exons
  • b) Junk DNA
  • c) Viral components
  • d) Introns
Q.10
Which of the following sequence must be removed from DNA expressed in Vaccinia vectors?
  • a) TTTTTNT
  • b) TTTTTTT
  • c) NNNNNNN
  • d) TNTNTNT
Q.11
A useful binary expression system contains Vaccinia virus and bacteriophage ________
  • a) T7
  • b) T5
  • c) T8
  • d) T9
Q.12
The vaccinia virus cannot be used to express antigens from other infectious agents.
  • a) True
  • b) False
Q.13
Which cells were transfected in an early demonstration that vaccinia virus could be used to express antigens from other infectious agents?
  • a) Human cells
  • b) Insect cells
  • c) Plant cells
  • d) Monkey cells
Q.14
Vaccinia viruses expressing the influenza hemagglutinin gene were used to immunize ____________
  • a) Hamsters
  • b) Humans
  • c) Rabbits
  • d) Cow
Q.15
Resistance to SIV and HIV-2 was shown by infecting _______ with Vaccinia vectors.
  • a) Cow
  • b) Plants
  • c) Drosophila
  • d) Mouse
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