Q.1
Agrobacterium tumefaciens can transfer DNA to animal cells.
  • a) True
  • b) False
Q.2
Which of the following is an artificial form of bactofection?
  • a) Transduction
  • b) Protoplast fusion
  • c) Bolistics
  • d) Electroporation
Q.3
In the cases of Salmonella species, lysis occurs in the __________
  • a) Outside the phagocytic vesicle
  • b) Inside the phagocytic vesicle
  • c) In lymphatic system
  • d) In nucleus
Q.4
Which of the following occurs in DNA transfer by A. Tumefaciens?
  • a) Cell lysis
  • b) Conjugation
  • c) Infection
  • d) Myeloma
Q.5
Pilus helps in the transfer of DNA through a process of ___________
  • a) Transduction
  • b) Transfection
  • c) Conjugation
  • d) Transformation
Q.6
Which is an important principle in gene transfer using live bacteria?
  • a) Amplification
  • b) Attenuation
  • c) Lysis
  • d) Phagocytosis
Q.7
What would happen if attenuated bacteria are not used for mammalian gene transfer?
  • a) Cell death
  • b) Tumor
  • c) Proliferation
  • d) Unsuccessful gene transfer
Q.8
Which of the following is a technique of bacterial attenuation?
  • a) Auxotrophic mutants
  • b) Removal of cell wall
  • c) Protoplast fusion
  • d) Conjugation
Q.9
AroA mutants are unable to synthesize _____________
  • a) Amino acids
  • b) Homeotic genes
  • c) Vitamins
  • d) Aromatic compounds
Q.10
Inducible autolysis is a technique for attenuation.
  • a) True
  • b) False
Q.11
Attenuation of ‘Listeria Monocytogenes’ can be achieved by ___________
  • a) Auxotrophic strains
  • b) Autolysis
  • c) Gene knockout
  • d) Mutation
Q.12
Apart from the introduction of DNA into cultured cells, bacteria mediated gene transfer has also been used for ________
  • a) Recombinant protein production
  • b) Transgenic animal
  • c) In vivo gene transfer
  • d) In vitro gene transfer
Q.13
Virus particles have a natural ability of ___________
  • a) Adsorbing on the cell surface
  • b) Proliferation
  • c) Inducing tumor
  • d) Inducing apoptosis
Q.14
Helper-independent vectors can _____________
  • a) Not Propagate
  • b) Propagate dependently
  • c) Propagate independently
  • d) Transduce the cell
Q.15
What happens if an essential gene is replaced in a viral vector?
  • a) Helper dependent
  • b) Helper independent
  • c) Non-function
  • d) Loss of transfection ability
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