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Vector Biology Gene Manipulation
Vector Biology Assessment
Quiz 1
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Q.1
Agrobacterium tumefaciens can transfer DNA to animal cells.
a) True
b) False
Q.2
Which of the following is an artificial form of bactofection?
a) Transduction
b) Protoplast fusion
c) Bolistics
d) Electroporation
Q.3
In the cases of Salmonella species, lysis occurs in the __________
a) Outside the phagocytic vesicle
b) Inside the phagocytic vesicle
c) In lymphatic system
d) In nucleus
Q.4
Which of the following occurs in DNA transfer by A. Tumefaciens?
a) Cell lysis
b) Conjugation
c) Infection
d) Myeloma
Q.5
Pilus helps in the transfer of DNA through a process of ___________
a) Transduction
b) Transfection
c) Conjugation
d) Transformation
Q.6
Which is an important principle in gene transfer using live bacteria?
a) Amplification
b) Attenuation
c) Lysis
d) Phagocytosis
Q.7
What would happen if attenuated bacteria are not used for mammalian gene transfer?
a) Cell death
b) Tumor
c) Proliferation
d) Unsuccessful gene transfer
Q.8
Which of the following is a technique of bacterial attenuation?
a) Auxotrophic mutants
b) Removal of cell wall
c) Protoplast fusion
d) Conjugation
Q.9
AroA mutants are unable to synthesize _____________
a) Amino acids
b) Homeotic genes
c) Vitamins
d) Aromatic compounds
Q.10
Inducible autolysis is a technique for attenuation.
a) True
b) False
Q.11
Attenuation of ‘Listeria Monocytogenes’ can be achieved by ___________
a) Auxotrophic strains
b) Autolysis
c) Gene knockout
d) Mutation
Q.12
Apart from the introduction of DNA into cultured cells, bacteria mediated gene transfer has also been used for ________
a) Recombinant protein production
b) Transgenic animal
c) In vivo gene transfer
d) In vitro gene transfer
Q.13
Virus particles have a natural ability of ___________
a) Adsorbing on the cell surface
b) Proliferation
c) Inducing tumor
d) Inducing apoptosis
Q.14
Helper-independent vectors can _____________
a) Not Propagate
b) Propagate dependently
c) Propagate independently
d) Transduce the cell
Q.15
What happens if an essential gene is replaced in a viral vector?
a) Helper dependent
b) Helper independent
c) Non-function
d) Loss of transfection ability
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