Q.1
EcoR1 has which of the following hexanucleotide recognition sequence?
  • a) GAATTC
  • b) GGATCC
  • c) AGATCT
  • d) GCAGCA
Q.2
Which of the following is an example of flush end cutter?
  • a) PvuI
  • b) PvuII
  • c) EcoR1
  • d) SfiI
Q.3
Which of the following does not produce cohesive ends upon cleavage on their recognition sequences?
  • a) EcoRI
  • b) PvuII
  • c) BamHI
  • d) HindIII
Q.4
What is dithiothreitol?
  • a) Reducing agent
  • b) Restriction endonuclease
  • c) Ligase
  • d) Exonuclease
Q.5
Restriction digests with which enzyme needs to be kept at a higher temperature than the usualdegree temperature?
  • a) TaqI
  • b) EcoRI
  • c) BamHI
  • d) HindIII
Q.6
How can it be determined that a DNA molecule is cleaved by the action of endonuclease?
  • a) Checking the viscosity of the solution
  • b) Temperature check
  • c) Color indication
  • d) pH check
Q.7
How can the sizes of individual cleavage products be determined?
  • a) Temperature check
  • b) Molecular weight check
  • c) Chromatography
  • d) Gel electrophoresis
Q.8
The rate of migration of a DNA molecule in an electrophoresis depends on which two factors?
  • a) Temperature, length
  • b) Length, source
  • c) Shape, charge-to-mass ratio
  • d) Shape, viscosity
Q.9
How many basepairs long DNA molecules can be distinguished by the polyacrylamide (40%) gel electrophoresis?
  • a) 1-100 kbp
  • b) 1-300 bp
  • c) 100-200 kbp
  • d) 300-600 bp
Q.10
What are double digestions?
  • a) Cutting with an enzyme twice on the same site
  • b) Cutting with two different enzymes on the same site
  • c) Cutting with an enzyme twice on different sites
  • d) Cutting with two different enzymes on the different sites
Q.11
What will happen if the incubation period, post restriction digestion is shortened?
  • a) Indigestion
  • b) Partial digestion
  • c) Double digestion
  • d) Complete digestion
Q.12
What does a complex pattern of bands in a gel electrophoresis signify?
  • a) Partial digestion
  • b) Double digestion
  • c) Complete digestion
  • d) Contaminated sample
Q.13
What is the full form of OFAGE?
  • a) Orthogonal field alternation gel electrophoresis
  • b) Orthogonal field attraction gel electrophoresis
  • c) Oligonucleotide field alternation gel electrophoresis
  • d) Oligonucleotide field attraction gel electrophoresis
Q.14
Which of the following techniques cannot be used to separate the genome of lower eukaryote yeast?
  • a) CHEF
  • b) OGAGE
  • c) FIGE
  • d) Gel electrophoresis
Q.15
FIGE is the modification of which technique?
  • a) OFAGE
  • b) Agarose gel electrophoresis
  • c) Electrophoresis
  • d) Acrylamide gel electrophoresis
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