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Vector Biology Gene Manipulation
Vector Biology Exam
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Q.1
What is reporter genes used for?
a) To confirm transformation
b) To confirm transfection
c) To induce expression
d) To cease expression
Q.2
What is “CAT”?
a) Repressor gene
b) Promoter
c) Reporter gene
d) Hybrid vector
Q.3
The CAT gene confers antibiotic resistance.
a) True
b) False
Q.4
Simian Virus(SVis an example of ____________
a) Caulimovirus
b) Polyomavirus
c) Plant virus
d) Retrovirus
Q.5
Polyomavirus vectors such as SVare used especially for ____________
a) Gene expression
b) Gene manipulation
c) Recombinant proteins
d) Hybrid vector production
Q.6
Which is the first virus to be developed as an animal vector?
a) Papillomavirus
b) SV40
c) Adenovirus
d) HIV
Q.7
What is the approximate size of the SVvector?
a) 1 kb
b) 3 kb
c) 5 kb
d) 7 kb
Q.8
Transcription in SVis controlled by ___________
a) Regulatory element
b) Late genes
c) Early genes
d) Promoter
Q.9
How many proteins are produced during the first stage of SVinfection cycle?
a) 1
b) 2
c) 3
d) 4
Q.10
What is the function of the T-antigen of SVviral vectors?
a) Genome replication
b) Translation
c) Transcription
d) Conjugation
Q.11
What function does an “oncoprotein” serve?
a) Controlled cell proliferation
b) Ceased cell proliferation
c) Uncontrolled cell proliferation
d) Initiates cell proliferation
Q.12
Which type of infection is caused by the human BK virus?
a) Viral
b) Lytic
c) Latent
d) Carcinogenic
Q.13
An epstein-barr virus causes a latent infection.
a) True
b) False
Q.14
What are polyadenylation signals?
a) Inducers
b) Terminators
c) Promoters
d) Replicons
Q.15
Which of the following is a source of Poly(A) sites, incorporated into mammalian vectors?
a) Mouse beta-globin
b) Bovine serum albumin
c) Maltose binding protein
d) Agrobacterium
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