Q.1
What is an additional feature of M13mp7?
  • a) 2 antibiotic resistance genes
  • b) Bigger size
  • c) Multiple cloning sites
  • d) Smaller size
Q.2
What is a phagemid?
  • a) A hybrid vector
  • b) Phage vector
  • c) Plasmid vector
  • d) Viral vector
Q.3
What does the Mfragment in a phagemid contain?
  • a) BamHI restriction site
  • b) Signal sequences
  • c) Origin of replication
  • d) Promoter sequence
Q.4
Why is a helper phage needed when cloning experiments which a hybrid vector such as Pembl8 is done?
  • a) Efficient insertion
  • b) Attachment to host
  • c) To provide replicative enzymes
  • d) Stable transformation
Q.5
How can the recombinants of phagemid vector Pembl8 be identified?
  • a) Agar plating
  • b) Agar + Antibiotic
  • c) Agar + X-gal
  • d) Minimal media plating
Q.6
What is the size of fragments that can be obtained by using a phagemid vector?
  • a) 1 kb
  • b) 10 kb
  • c) 1500 bp
  • d) 50 kb
Q.7
What is the size limit for in vitro packaging of an unmodified lambda vector?
  • a) 10 kb
  • b) 52 kb
  • c) 100 kb
  • d) 10 bp
Q.8
Which non-essential region of the lambda phage can be deleted without impairing viability?
  • a) Protein coding
  • b) Promoter region
  • c) Integration and excision region
  • d) Terminator region
Q.9
What is the basic difference between a modified (non-essential regions removed) and an unmodified lambda vector?
  • a) Gene expression increases
  • b) Stable infection
  • c) Non- lysogenic cycle
  • d) Star activity
Q.10
Why is natural selection used to isolate modified lambda that lacks certain restriction sites?
  • a) Strains that lack sites are known
  • b) Easier than in vitro mutagenesis
  • c) There are no restriction sites in lambda
  • d) Natural selection is less time consuming
Q.11
Why is natural selection used to isolate modified lambda that lacks certain restriction sites?
  • a) Strains that lack sites are known
  • b) Easier than in vitro mutagenesis
  • c) There are no restriction sites in lambda
  • d) Natural selection is less time consuming
Q.12
Insertion and replacement vectors are modified vectors of which of the following?
  • a) Plasmid
  • b) Lambda phage
  • c) M13 phage
  • d) Yeast artificial chromosome
Q.13
What type of vector is the lambda-gt10?
  • a) Insertion vector
  • b) Replacement vector
  • c) Hybrid vector
  • d) Unmodified lambda vector
Q.14
Which property is not associated with a lambda insertion vector?
  • a) Non-essential region removed
  • b) Two vector arms ligated together
  • c) At least one restriction site is present
  • d) Expression of the gene can be obtained
Q.15
How can identification of recombinants of lambda-gtvector be done?
  • a) Ampicillin resistance
  • b) Lac selection
  • c) cI gene insertional inactivation
  • d) Agar and X-gal plating
0 h : 0 m : 1 s