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Vector Biology Gene Manipulation
Vector Biology Multiple Choice
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Q.1
What is nisin?
a) Inducer
b) Protein
c) Promoter
d) Suppressor
Q.2
The export system of E. coli is ________ to that in mammals.
a) Different
b) Similar
c) Larger
d) Smaller
Q.3
Two probe vectors were developed for the identification of secretion signals. These incorporated a gene from ___________
a) Streptococcus
b) Bacillus
c) Pseudomonas
d) Parasitoid
Q.4
In probe vectors that identify secret signals, the reporter gene codes for___________
a) Lactose
b) Beta amylase
c) Alpha amylase
d) Amylase
Q.5
Iodine used for the detection of secreted amylase ________ the bacteria.
a) Inactivates
b) Kills
c) Produces
d) Grows
Q.6
Which media is used in the culture of probe detection vectors?
a) Starch
b) Glucose
c) Lactose
d) Pectin
Q.7
To avoid killing of bacterial colonies in the iodine detection assay, which of the following techniques can be used?
a) Ligation
b) Dissection
c) Cloning
d) Replica Plating
Q.8
The S. aureus-secreted nuclease is ______ amino acids long.
a) 100
b) 168
c) 208
d) 68
Q.9
The S. aureus secreted nuclease is dimeric.
a) True
b) False
Q.10
Inclusion of a synthetic polypeptide in probe vector system can ________________
a) Enhance secretion
b) Decrease secretion
c) Decrease size
d) Increase size
Q.11
To understand gene function, vectors have been developed for directed _________________
a) Mutagenesis
b) Protein production
c) Gene activation
d) Cancerous growth
Q.12
P-MUTIN are the vectors that perform directed insertional mutation in _________________
a) S. aureus
b) Saccharomyces
c) B. subtilis
d) Pseudomonas
Q.13
Which reporter gene is used in P-MUTIN vectors?
a) Amp-R
b) LacZ
c) Tet-R
d) Gal
Q.14
Which promoter is used in P-MUTIN vectors?
a) Lac
b) Gal
c) Tol
d) Psac
Q.15
The Psac promoter in P-MUTIN vectors is inducible.
a) True
b) False
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