Q.1
What is nisin?
  • a) Inducer
  • b) Protein
  • c) Promoter
  • d) Suppressor
Q.2
The export system of E. coli is ________ to that in mammals.
  • a) Different
  • b) Similar
  • c) Larger
  • d) Smaller
Q.3
Two probe vectors were developed for the identification of secretion signals. These incorporated a gene from ___________
  • a) Streptococcus
  • b) Bacillus
  • c) Pseudomonas
  • d) Parasitoid
Q.4
In probe vectors that identify secret signals, the reporter gene codes for___________
  • a) Lactose
  • b) Beta amylase
  • c) Alpha amylase
  • d) Amylase
Q.5
Iodine used for the detection of secreted amylase ________ the bacteria.
  • a) Inactivates
  • b) Kills
  • c) Produces
  • d) Grows
Q.6
Which media is used in the culture of probe detection vectors?
  • a) Starch
  • b) Glucose
  • c) Lactose
  • d) Pectin
Q.7
To avoid killing of bacterial colonies in the iodine detection assay, which of the following techniques can be used?
  • a) Ligation
  • b) Dissection
  • c) Cloning
  • d) Replica Plating
Q.8
The S. aureus-secreted nuclease is ______ amino acids long.
  • a) 100
  • b) 168
  • c) 208
  • d) 68
Q.9
The S. aureus secreted nuclease is dimeric.
  • a) True
  • b) False
Q.10
Inclusion of a synthetic polypeptide in probe vector system can ________________
  • a) Enhance secretion
  • b) Decrease secretion
  • c) Decrease size
  • d) Increase size
Q.11
To understand gene function, vectors have been developed for directed _________________
  • a) Mutagenesis
  • b) Protein production
  • c) Gene activation
  • d) Cancerous growth
Q.12
P-MUTIN are the vectors that perform directed insertional mutation in _________________
  • a) S. aureus
  • b) Saccharomyces
  • c) B. subtilis
  • d) Pseudomonas
Q.13
Which reporter gene is used in P-MUTIN vectors?
  • a) Amp-R
  • b) LacZ
  • c) Tet-R
  • d) Gal
Q.14
Which promoter is used in P-MUTIN vectors?
  • a) Lac
  • b) Gal
  • c) Tol
  • d) Psac
Q.15
The Psac promoter in P-MUTIN vectors is inducible.
  • a) True
  • b) False
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