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Vector Biology Gene Manipulation
Vector Biology Objective
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Q.1
Why is AAV regarded as one of the safest vectors for gene therapy?
a) Lack origin
b) Helper virus dependent
c) Small sized genome
d) No toxic effects
Q.2
Where is foreign DNA inserted in an AAV virus vector?
a) Cap region
b) Rep region
c) Inverted repeats
d) Origin of replication
Q.3
Which is the only element essential for replication in an AAV vector?
a) Origin of replication
b) Inverted repeats
c) Rep genes
d) Cap genes
Q.4
Either endogenous or homologous promoters can be used with an AAV vector.
a) True
b) False
Q.5
Deletion of which region of an AAV vector abolishes the site-specificity?
a) Origin of replication
b) Cap region
c) Rep region
d) Inverted repeats
Q.6
Which of the following type of cells cannot be transfected by AAV vector?
a) Liver cells
b) Germ cells
c) Muscle cells
d) Neurons
Q.7
Use of con-catemeric replication intermediates, circumvents which of the most serious disadvantage of AAV vectors?
a) Replication ability
b) Infection cycle
c) DNA carrying capacity
d) Host range
Q.8
‘Nuclear occlusion bodies’ are found in _______________
a) Baculoviruses
b) Adenovirus
c) Human somatic cells
d) Human germ cells
Q.9
Nuclear occlusion bodies are _____________
a) Nuclear particles
b) Vitamins
c) Proteinaceous particles
d) Replication genes
Q.10
Which protein is expressed at high levels in occlusion bodies of baculoviruses?
a) Polyhedron
b) Amylose
c) Glucose
d) Lactose
Q.11
The polyhedrin gene of baculovirus vectors cannot be replaced by foreign DNA.
a) True
b) False
Q.12
AcMNPV vector is a type of __________ which is used for expression in insect cells.
a) Baculoviruses
b) Adenoviruses
c) Caulimoviruses
d) Papillomavirus
Q.13
Bombyx mori nuclear polyhedrosis virus infects the _______
a) Drosophila
b) Plants
c) Humans
d) Silkworm
Q.14
One limitation of baculovirus expressed system is that ___________ in mammals and insects is different.
a) Glycosylation pathway
b) Genomic size
c) Cloning efficiency
d) Virus genre
Q.15
Insect cell lines derived from Estigmene area have the ability of __________
a) Post-translational modification
b) Replication
c) Transduction
d) Conjugation
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