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Vector Biology Gene Manipulation
Vector Biology Written Test
Quiz 1
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Q.1
What does the replicase protein of alphaviral vectors produce?
a) Transgene
b) Complementary strand
c) Daughter genome
d) Host genome
Q.2
The negative strand produced by replicase protein contains no internal promoter.
a) True
b) False
Q.3
Replication competitive vectors contain an additional __________
a) Promoter
b) Subgenomic promoter
c) Inducer
d) Double promoter
Q.4
Introduction of foreign DNA downstream of the subgenomic promoter produces two distinct _________________
a) RNAs
b) Proteins
c) DNA
d) Transgene
Q.5
In insertion alphaviral vectors, the capsid polyprotein is replaced by __________
a) Promoter
b) Inducer
c) RNA
d) Transgene
Q.6
A strong enhancer of protein synthesis in the Sindbis vector increases ______________
a) Recombinant protein
b) Recombinant DNA
c) Transgene
d) Genomic size
Q.7
Why is the enhancer region included in many vectors based on alphaviruses?
a) Expression of protein on N-terminus
b) Expression of protein on P-terminus
c) Expression as a fusion protein
d) To decrease expression
Q.8
pSinRep5 is a ________ vector marketed by Invitrogen.
a) Sindbis replicon
b) Hybrid
c) Alphavirus
d) Baculovirus
Q.9
Which of the following promoters is used in pSinRep5 vector?
a) SP2
b) SP4
c) SP6
d) SP8
Q.10
What is the function of downstream restriction sites of the pSinRep5 vector?
a) Circularization
b) Linearization
c) DNA replication
d) Transfection
Q.11
Which of the following is a standard eukaryotic promoter?
a) SV10
b) SV20
c) SV30
d) SV40
Q.12
Transduction is a more suitable procedure for gene therapy using alphavirus vectors.
a) True
b) False
Q.13
Vaccinia is a type of ___________
a) Poxvirus
b) Hybrid vector
c) Alphavirus
d) Vaccination
Q.14
What is the unusual property associated with poxviruses?
a) Small size
b) Replication mechanism
c) Expression mechanism
d) Replication site
Q.15
What is the approximate size of poxviruses?
a) 100 kb
b) 200 kb
c) 300 kb
d) 10 kb
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