Q.1
What does the replicase protein of alphaviral vectors produce?
  • a) Transgene
  • b) Complementary strand
  • c) Daughter genome
  • d) Host genome
Q.2
The negative strand produced by replicase protein contains no internal promoter.
  • a) True
  • b) False
Q.3
Replication competitive vectors contain an additional __________
  • a) Promoter
  • b) Subgenomic promoter
  • c) Inducer
  • d) Double promoter
Q.4
Introduction of foreign DNA downstream of the subgenomic promoter produces two distinct _________________
  • a) RNAs
  • b) Proteins
  • c) DNA
  • d) Transgene
Q.5
In insertion alphaviral vectors, the capsid polyprotein is replaced by __________
  • a) Promoter
  • b) Inducer
  • c) RNA
  • d) Transgene
Q.6
A strong enhancer of protein synthesis in the Sindbis vector increases ______________
  • a) Recombinant protein
  • b) Recombinant DNA
  • c) Transgene
  • d) Genomic size
Q.7
Why is the enhancer region included in many vectors based on alphaviruses?
  • a) Expression of protein on N-terminus
  • b) Expression of protein on P-terminus
  • c) Expression as a fusion protein
  • d) To decrease expression
Q.8
pSinRep5 is a ________ vector marketed by Invitrogen.
  • a) Sindbis replicon
  • b) Hybrid
  • c) Alphavirus
  • d) Baculovirus
Q.9
Which of the following promoters is used in pSinRep5 vector?
  • a) SP2
  • b) SP4
  • c) SP6
  • d) SP8
Q.10
What is the function of downstream restriction sites of the pSinRep5 vector?
  • a) Circularization
  • b) Linearization
  • c) DNA replication
  • d) Transfection
Q.11
Which of the following is a standard eukaryotic promoter?
  • a) SV10
  • b) SV20
  • c) SV30
  • d) SV40
Q.12
Transduction is a more suitable procedure for gene therapy using alphavirus vectors.
  • a) True
  • b) False
Q.13
Vaccinia is a type of ___________
  • a) Poxvirus
  • b) Hybrid vector
  • c) Alphavirus
  • d) Vaccination
Q.14
What is the unusual property associated with poxviruses?
  • a) Small size
  • b) Replication mechanism
  • c) Expression mechanism
  • d) Replication site
Q.15
What is the approximate size of poxviruses?
  • a) 100 kb
  • b) 200 kb
  • c) 300 kb
  • d) 10 kb
0 h : 0 m : 1 s